RNA-sequencing analysis of umbilical cord plasma microRNAs from healthy newborns

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dc.contributor.author Brennan, Gary P.
dc.contributor.author Vitsios, Dimitrios M.
dc.contributor.author Casey, Sophie
dc.contributor.author Looney, Ann-Marie
dc.contributor.author Hallberg, Boubou
dc.contributor.author Henshall, David C.
dc.contributor.author Boylan, Geraldine B.
dc.contributor.author Murray, Deirdre M.
dc.contributor.author Mooney, Catherine
dc.date.accessioned 2019-06-24T13:40:01Z
dc.date.available 2019-06-24T13:40:01Z
dc.date.issued 2018-12-03
dc.identifier.citation Brennan, G.P., Vitsios, D.M., Casey, S., Looney, A.M., Hallberg, B., Henshall, D.C., Boylan, G.B., Murray, D.M. and Mooney, C., 2018. RNA-sequencing analysis of umbilical cord plasma microRNAs from healthy newborns. PLoS ONE 13(12): e020795, (26pp.) DOI: 10.1371/journal.pone.0207952 en
dc.identifier.volume 13 en
dc.identifier.issued 12 en
dc.identifier.startpage 1 en
dc.identifier.endpage 26 en
dc.identifier.uri http://hdl.handle.net/10468/8100
dc.identifier.doi 10.1371/journal.pone.0207952 en
dc.description.abstract MicroRNAs are a class of small non-coding RNA that regulate gene expression at a posttranscriptional level. MicroRNAs have been identified in various body fluids under normal conditions and their stability as well as their dysregulation in disease has led to ongoing interest in their diagnostic and prognostic potential. Circulating microRNAs may be valuable predictors of early-life complications such as birth asphyxia or neonatal seizures but there are relatively few data on microRNA content in plasma from healthy babies. Here we performed small RNA-sequencing analysis of plasma processed from umbilical cord blood in a set of healthy newborns. MicroRNA levels in umbilical cord plasma of four male and four female healthy babies, from two different centres were profiled. A total of 1,004 individual microRNAs were identified, which ranged from 426 to 659 per sample, of which 269 microRNAs were common to all eight samples. Many of these microRNAs are highly expressed and consistent with previous studies using other high throughput platforms. While overall microRNA expression did not differ between male and female cord blood plasma, we did detect differentially edited microRNAs in female plasma compared to male. Of note, and consistent with other studies of this type, adenylation and uridylation were the two most prominent forms of editing. Six microRNAs, miR-128-3p, miR-29a-3p, miR-9-5p, miR-218-5p, 204-5p and miR-132-3p were consistently both uridylated and adenylated in female cord blood plasma. These results provide a benchmark for microRNA profiling and biomarker discovery using umbilical cord plasma and can be used as comparative data for future biomarker profiles from complicated births or those with earlylife developmental disorders en
dc.description.sponsorship Science Foundation Ireland (SFI/16/RC/3948; SFI/12/COEN/18) ; Health Research Board (HRB Clinician Scientist Award CSA 2012/40) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLoS ONE en
dc.relation.uri https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0207952
dc.rights © 2018 Brennan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject MicroRNA en
dc.subject Gene expression en
dc.subject RNA sequencing analysis en
dc.subject Umbilical cord plasma en
dc.title RNA-sequencing analysis of umbilical cord plasma microRNAs from healthy newborns en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Geraldine Boylan, Department of Medicine, University College Cork, Cork, Ireland. +353-21-490-3000 Email: g.boylan@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder European Regional Development Fund en
dc.contributor.funder Royal College of Surgeons in Ireland en
dc.contributor.funder Epilepsy Ireland en
dc.contributor.funder Irish Research Council en
dc.contributor.funder European Molecular Biology Laboratory en
dc.contributor.funder Medical Research Charities Group en
dc.contributor.funder FutureNeuro en
dc.contributor.funder Health Research Board en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS ONE en
dc.internal.IRISemailaddress g.boylan@ucc.ie en
dc.identifier.articleid e0207952 en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2272/IE/Irish Centre for Fetal and Neonatal Translational Research (INFANT)/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1891/IE/MicroRNA biofluid profiles as molecular diagnostics for epilepsy/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Advance Award Programme/14/ADV/RC2721/IE/Bioinformatics and predictive analytics for the identification of microRNA biomarkers of neonatal seizures/ en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::MSCA-IF-EF-RI/707530/EU/A comprehensive analysis of microRNA-124 regulated gene networks and its role in epilepsy./EpiMIRgen en
dc.identifier.eissn 1932-6203


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© 2018 Brennan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2018 Brennan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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