Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota

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dc.contributor.author Riboulet-Bisson, Eliette
dc.contributor.author Sturme, Mark H. J.
dc.contributor.author Jeffery, Ian B.
dc.contributor.author O'Donnell, Michelle M.
dc.contributor.author Neville, B. Anne
dc.contributor.author Forde, Brian M.
dc.contributor.author Claesson, Marcus J.
dc.contributor.author Harris, Hugh Michael B.
dc.contributor.author Gardiner, Gillian E.
dc.contributor.author Casey, Pat G.
dc.contributor.author Lawlor, Peadar G.
dc.contributor.author O'Toole, Paul W.
dc.contributor.author Ross, R. Paul
dc.contributor.editor Dias-Neto, Emmanuel
dc.date.accessioned 2012-11-29T12:45:36Z
dc.date.available 2012-11-29T12:45:36Z
dc.date.copyright 2012
dc.date.issued 2012-02-17
dc.identifier.citation RIBOULET-BISSON, E., STURME, M. H. J., JEFFERY, I. B., O'DONNELL, M. M., NEVILLE, B. A., FORDE, B. M., CLAESSON, M. J., HARRIS, H., GARDINER, G. E., CASEY, P. G., LAWLOR, P. G., O'TOOLE, P. W. & ROSS, R. P. 2012. Effect of Lactobacillus salivarius Bacteriocin Abp118 on the Mouse and Pig Intestinal Microbiota. Plos One, 7, e31113. doi:10.1371/journal.pone.0031113 en
dc.identifier.volume 7 en
dc.identifier.issued 2 en
dc.identifier.startpage e31113 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/827
dc.identifier.doi 10.1371/journal.pone.0031113
dc.description.abstract Lactobacilli are Gram-positive bacteria that are a subdominant element in the human gastrointestinal microbiota, and which are commonly used in the food industry. Some lactobacilli are considered probiotic, and have been associated with health benefits. However, there is very little culture-independent information on how consumed probiotic microorganisms might affect the entire intestinal microbiota. We therefore studied the impact of the administration of Lactobacillus salivarius UCC118, a microorganism well characterized for its probiotic properties, on the composition of the intestinal microbiota in two model animals. UCC118 has anti-infective activity due to production of the bacteriocin Abp118, a broad-spectrum class IIb bacteriocin, which we hypothesized could impact the microbiota. Mice and pigs were administered wild-type (WT) L. salivarius UCC118 cells, or a mutant lacking bacteriocin production. The microbiota composition was determined by pyrosequencing of 16S rRNA gene amplicons from faeces. The data show that L. salivarius UCC118 administration had no significant effect on proportions of major phyla comprising the mouse microbiota, whether the strain was producing bacteriocin or not. However, L. salivarius UCC118 WT administration led to a significant decrease in Spirochaetes levels, the third major phylum in the untreated pig microbiota. In both pigs and mice, L. salivarius UCC118 administration had an effect on Firmicutes genus members. This effect was not observed when the mutant strain was administered, and was thus associated with bacteriocin production. Surprisingly, in both models, L. salivarius UCC118 administration and production of Abp118 had an effect on Gram-negative microorganisms, even though Abp118 is normally not active in vitro against this group of microorganisms. Thus L. salivarius UCC118 administration has a significant but subtle impact on mouse and pig microbiota, by a mechanism that seems at least partially bacteriocin-dependent. en
dc.description.sponsorship Science Foundation Ireland (Principal Investigator award (07/IN.1/B1780)); Irish Research Council for Science Engineering and Technology (Embark Studentship) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher PLOS en
dc.relation.uri http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0031113
dc.rights © 2012 Riboulet-Bisson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri http://creativecommons.org/licenses/by/2.5/ en
dc.subject Lactobacillus salivarius en
dc.subject Bacteriocin Abp118 en
dc.subject Spirochaetes en
dc.subject Intestinal microbiota en
dc.subject 16S rRNA en
dc.subject Gram-negative microorganisms en
dc.title Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://research.ucc.ie/profiles/D010/pwotoole en
dc.internal.authorcontactother Paul O'Toole, Department of Microbiology, University College Cork, Cork, Ireland E-mail: pwotoole@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.internal.rssid 145963142
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Irish Research Council for Science Engineering and Technology en
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS ONE en
dc.internal.copyrightchecked Sherpa Romeo Green Journal. Policy: The Public Library of Science (PLOS) applies the Creative Commons Attribution License (CC-BY) to works we publish (read the human-readable summary or the full license legal code). Under this license, authors retain ownership of the copyright for their content, but allow anyone to download, reuse, reprint, modify, distribute, and/or copy the content as long as the original authors and source are cited. No permission is required from the authors or the publishers. Appropriate attribution can be provided by simply citing the original article (e.g., Kaltenbach LS et al. (2007) Huntingtin Interacting Proteins Are Genetic Modifiers of Neurodegeneration. PLOS Genet 3(5): e82. doi:10.1371/journal.pgen.0030082). For any reuse or redistribution of a work, users must also make clear the license terms under which the work was published. en
dc.internal.IRISemailaddress pwotoole@ucc.ie en


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© 2012 Riboulet-Bisson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2012 Riboulet-Bisson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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