Mechanisms of adherence of a probiotic Lactobacillus strain during and after in vivo assessment in ulcerative colitis patients

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dc.contributor.author Dunne, Colum P.
dc.contributor.author Kelly, Peter
dc.contributor.author O'Halloran, Sile
dc.contributor.author Soden, Declan
dc.contributor.author Bennett, Mary
dc.contributor.author von Wright, Atte
dc.contributor.author Vilpponen-Salmela, Terttu
dc.contributor.author Kiely, Barry
dc.contributor.author O'Mahony, Liam
dc.contributor.author Collins, J. Kevin
dc.contributor.author O'Sullivan, Gerald C.
dc.contributor.author Shanahan, Fergus
dc.date.accessioned 2012-12-10T10:21:14Z
dc.date.available 2012-12-10T10:21:14Z
dc.date.copyright 2004
dc.date.issued 2004-07
dc.identifier.citation DUNNE, C., KELLY, P., O'HALLORAN, S., SODEN, D., BENNETT, M., VON WRIGHT, A., VILPPONEN-SALMELA, T., KIELY, B., O'MAHONY, L., COLLINS, J. K., O'SULLIVAN, G. C. & SHANAHAN, F. 2004. Mechanisms of adherence of a probiotic Lactobacillus strain during and after in vivo assessment in ulcerative colitis patients. Microb Ecol Health Dis, 16, 96-104. en
dc.identifier.volume 16 en
dc.identifier.issued 2/3 en
dc.identifier.startpage 96 en
dc.identifier.endpage 104 en
dc.identifier.issn 1651-2235
dc.identifier.issn 0891-060X
dc.identifier.uri http://hdl.handle.net/10468/839
dc.identifier.doi 10.1080/08910600410032295.
dc.description.abstract In a pilot-scale, open-label study to determine the ability of well-characterized probiotic Lactobacillus salivarius UCC118 cells to adhere to human epithelial cells in situ , the bacterial strain was administered to ulcerative colitis patients at approximately 109 CFU/day for 12 days. Microbiological analysis of biopsy specimens demonstrated that the ingested bacteria effectively adhered to both inflamed and non-inflamed mucosa of the large bowel in significant numbers. In previous reports, we have described the ability of the lactobacilli to adhere to enterocytic epithelial cells in vitro. In this study, we found that the bacteria adhered at higher levels to differentiated rather than undifferentiated epithelial monolayers; and that stationary phase lactobacilli were found to adhere to eukaryotic HT-29 and Caco-2 epithelial cells at greater levels than log phase bacterial cells. Pretreatment of the Lactobacillus cells with proteolytic enzymes abolished attachment, indicating the potential involvement of surface/exposed protein(s) as bacterial adhesin(s). SDS-PAGE (denaturing) techniques determined that the proteolytic treatment resulted in degradation of a cell wall-associated protein of approximately 84 kDa. The proteinaceous factor was purified by both anion-exchange chromatography and by gel extraction after SDS-PAGE electrophoresis, and under in vitro assay conditions proved capable of adherence and significant inhibition of bacterial attachment to enterocytic epithelial cells. Key words: probiotic, Lactobacillus, adhesin, cell-borne, proteinaceous. en
dc.description.sponsorship Department of Agriculture and Food, Ireland (Food Sub-Programme of the Operational Programme for Industrial Development); Higher Education Authority (Programme for Research in Third Level Institutions (PRTLI)); European Commission (PROBDEMO: FAIR-CT96-1028, PROGID: QLK1-2000-00563) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Co-Action Publishing en
dc.rights.uri http://creativecommons.org/licenses/by/3.0/us/ en
dc.subject Probiotic en
dc.subject Lactobacillus en
dc.subject Adhesin en
dc.subject Cell-borne en
dc.subject Proteinaceous en
dc.title Mechanisms of adherence of a probiotic Lactobacillus strain during and after in vivo assessment in ulcerative colitis patients en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://research.ucc.ie/profiles/C012/fshanahan en
dc.internal.authorcontactother Fergus Shanahan, Alimentary Pharmabiotic Centre, Biosciences Building, University College Cork, Cork, Ireland, E-mail: f.shanahan@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder European Regional Development Fund en
dc.contributor.funder Department of Agriculture and Food, Ireland en
dc.contributor.funder Health Research Board en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder European Commission en
dc.contributor.funder Higher Education Authority en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Microbial Ecology in Health and Disease en
dc.internal.copyrightchecked Sherpa Romeo Green Journal. Policy: Co-Action Publishing requires that authors publish their work under the Creative Commons Attribution-NonCommercial 3.0 Unported license. This allows third parties to share your article or book (copy, distribute, transmit) and to adapt it, under the condition that the work is attributed to in the manner specified by you, that it is not used for commercial purposes, and that in the event of reuse or distribution, the terms of this license are made clear. en
dc.internal.IRISemailaddress f.shanahan@ucc.ie en
dc.internal.IRISemailaddress peter.kelly@ucc.ie en


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