A yeast-based biosensor for screening of short- and medium-chain fatty acid production

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dc.contributor.author Baumann, Leonie
dc.contributor.author Rajkumar, Arun S.
dc.contributor.author Morrissey, John P.
dc.contributor.author Boles, Eckhard
dc.contributor.author Oreb, Mislav
dc.date.accessioned 2019-09-02T14:36:33Z
dc.date.available 2019-09-02T14:36:33Z
dc.date.issued 2018-10-19
dc.identifier.citation Baumann, L., Rajkumar, A.S., Morrissey, J.P., Boles, E. and Oreb, M., 2018. A Yeast-Based Biosensor for Screening of Short-and Medium-Chain Fatty Acid Production. ACS synthetic biology, 7(11), (7pp). DOI:10.1021/acssynbio.8b00309 en
dc.identifier.volume 7 en
dc.identifier.issued 11 en
dc.identifier.startpage 2640 en
dc.identifier.endpage 2646 en
dc.identifier.issn 2161-5063
dc.identifier.uri http://hdl.handle.net/10468/8434
dc.identifier.doi 10.1021/acssynbio.8b00309 en
dc.description.abstract Short- and medium-chain fatty acids (SMCFA) are important platform chemicals currently produced from nonsustainable resources. The engineering of microbial cells to produce SMCFA, however, lacks high-throughput methods to screen for best performing cells. Here, we present the development of a whole-cell biosensor for easy and rapid detection of SMCFA. The biosensor is based on a multicopy yeast plasmid containing the SMCFA-responsive PDR12 promoter coupled to GFP as the reporter gene. The sensor detected hexanoic, heptanoic and octanoic acid over a linear range up to 2, 1.5, and 0.75 mM, respectively, but did not show a linear response to decanoic and dodecanoic acid. We validated the functionality of the biosensor with culture supernatants of a previously engineered Saccharomyces cerevisiae octanoic acid producer strain and derivatives thereof. The biosensor signal correlated strongly with the octanoic acid concentrations as determined by gas chromatography. Thus, this biosensor enables the high-throughput screening of SMCFA producers and has the potential to drastically speed up the engineering of diverse SMCFA producing cell factories. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Chemical Society en
dc.relation.uri https://pubs.acs.org/doi/10.1021/acssynbio.8b00309
dc.rights © 2018 American Chemical Society en
dc.rights.uri http://www.copyright.com/ en
dc.subject Biosensor en
dc.subject High-throughput screening en
dc.subject Short-chain fatty acids en
dc.subject Medium-chain fatty acids en
dc.subject Octanoic acid en
dc.subject PDR12 en
dc.title A yeast-based biosensor for screening of short- and medium-chain fatty acid production en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John Morrissey, APC Microbiome Institute, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.morrissey@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Horizon 2020 en
dc.description.status Peer reviewed en
dc.identifier.journaltitle ACS Synthetic Biology en
dc.internal.IRISemailaddress j.morrissey@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::RIA/720824/EU/Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds/CHASSY en
dc.identifier.eissn 2161-5063

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