Functional characterization of the Lactolisterin BU gene cluster of Lactococcus lactis subsp. lactis BGBU1-4

Show simple item record

dc.contributor.author Miljkovic, Marija
dc.contributor.author Lozo, Jelena
dc.contributor.author Mirkovic, Nemanja
dc.contributor.author O'Connor, Paula M.
dc.contributor.author Malesevic, Milka
dc.contributor.author Jovcic, Branko
dc.contributor.author Cotter, Paul D.
dc.contributor.author Kojic, Milan
dc.date.accessioned 2019-09-04T08:17:12Z
dc.date.available 2019-09-04T08:17:12Z
dc.date.issued 2018-11-15
dc.identifier.citation Miljković, M., Lozo, J., Mirkovic, N., O'Connor, P.M., Malesevic, M., Jovčić, B., Cotter, P.D. and Kojic, M., 2018. Functional Characterization of the Lactolisterin BU Gene Cluster of Lactococcus lactis subsp. lactis BGBU1-4. Frontiers in microbiology, 9, (2774). DOI: 10.3389/fmicb.2018.02774 en
dc.identifier.volume 9 en
dc.identifier.startpage 1 en
dc.identifier.endpage 15 en
dc.identifier.uri http://hdl.handle.net/10468/8444
dc.identifier.doi 10.3389/fmicb.2018.02774 en
dc.description.abstract The gene cluster responsible for the production of the aureocin A53-like bacteriocin, lactolisterin BU, is located on plasmid pBU6 in Lactococcus lactis subsp. lactis BGBU1- 4. Heterologous expression of pBU6 confirmed that production and limited immunity to lactolisterin BU were provided by the plasmid. Comparative analysis of aureocin A53- like operons revealed that the structural genes shared a low level of identity, while other genes were without homology, indicating a different origin. Subcloning and expression of genes located downstream of the structural gene, lliBU, revealed that the lactolisterin BU cluster consists of four genes: the structural gene lliBU, the abcT gene encoding an ABC transporter, the accL gene encoding an accessory protein and the immL gene which provides limited immunity to lactolisterin BU. Reverse transcription analysis revealed that all genes were transcribed as one polycistronic mRNA. Attempts to split the lactolisterin BU operon, even when both parts were under control of the PlliBU promoter, were unsuccessful indicate g that expression of lactolisterin BU is probably precisely regulated at the translational level by translational coupling and is possible only when all genes of the operon are in cis constellation. Two ρ-independent transcription terminators were detected in the lactolisterin BU operon: the first in the intergenic region of the lliBU and abcT genes and the second at the end of operon. Deletion of the second transcription terminator did not influence production of the bacteriocin in lactococci. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fmicb.2018.02774/full
dc.rights © 2018 Miljkovic, Lozo, Mirkovic, O’Connor, Malesevic, Jovcic, Cotter and Kojic en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Bacteriocin en
dc.subject Lactolisterin BU operon en
dc.subject ABC transporter en
dc.subject Limited immunit en
dc.subject Translational coupling en
dc.title Functional characterization of the Lactolisterin BU gene cluster of Lactococcus lactis subsp. lactis BGBU1-4 en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Paula O'Connor, APC Microbiome Ireland, University College Cork, Cork, Ireland. +353-21-490-3000 en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Microbiology en
dc.identifier.articleid 2774 en
dc.relation.project info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS/Genes and molecular mechanisms promoting probiotic activity of lactic acid bacteria from Western Balkan/ en
dc.identifier.eissn 1664-302X


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2018 Miljkovic, Lozo, Mirkovic, O’Connor, Malesevic, Jovcic, Cotter and Kojic Except where otherwise noted, this item's license is described as © 2018 Miljkovic, Lozo, Mirkovic, O’Connor, Malesevic, Jovcic, Cotter and Kojic
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement