Pregnancy-specific glycoprotein expression in normal gastrointestinal tract and in tumors detected with novel monoclonal antibodies.

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dc.contributor.author Houston, Aileen M.
dc.contributor.author Williams, John M.
dc.contributor.author Rovis, Tihana L.
dc.contributor.author Shanley, Daniel K.
dc.contributor.author O'Riordan, Ronan T.
dc.contributor.author Kiely, Patrick A.
dc.contributor.author Ball, Melanie
dc.contributor.author Barry, Orla P.
dc.contributor.author Kelly, Jacquie
dc.contributor.author Fanning, Aine
dc.contributor.author MacSharry, John
dc.contributor.author Mandelboim, Ofer
dc.contributor.author Singer, Bernhard B.
dc.contributor.author Jonjic, Stipan
dc.contributor.author Moore, Thomas F.
dc.date.accessioned 2019-09-04T15:19:52Z
dc.date.available 2019-09-04T15:19:52Z
dc.date.issued 2016-03-18
dc.identifier.citation Houston, A., Williams, J. M., Rovis, T. L., Shanley, D. K., O'Riordan, R. T., Kiely, P. A., Ball, M., Barry, O. P., Kelly, J., Fanning, A., MacSharry, J., Mandelboim, O., Singer, B. B., Jonjic, S. and Moore, T. (2016) 'Pregnancy-specific glycoprotein expression in normal gastrointestinal tract and in tumors detected with novel monoclonal antibodies', mAbs, 8(3), pp. 491-500. doi: 10.1080/19420862.2015.1134410 en
dc.identifier.volume 8 en
dc.identifier.issued 3 en
dc.identifier.startpage 491 en
dc.identifier.endpage 500 en
dc.identifier.issn 1942-0870
dc.identifier.uri http://hdl.handle.net/10468/8453
dc.identifier.doi 10.1080/19420862.2015.1134410 en
dc.description.abstract Pregnancy-specific glycoproteins (PSGs) are immunoglobulin superfamily members related to the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) family and are encoded by 10 genes in the human. They are secreted at high levels by placental syncytiotrophoblast into maternal blood during pregnancy, and are implicated in immunoregulation, thromboregulation, and angiogenesis. To determine whether PSGs are expressed in tumors, we characterized 16 novel monoclonal antibodies to human PSG1 and used 2 that do not cross-react with CEACAMs to study PSG expression in tumors and in the gastrointestinal (GI) tract using tissue arrays and immunohistochemistry. Staining was frequently observed in primary squamous cell carcinomas and colonic adenocarcinomas and was correlated with the degree of tumor differentiation, being largely absent from metastatic samples. Staining was also observed in normal oesophageal and colonic epithelium. PSG expression in the human and mouse GI tract was confirmed using quantitative RT-PCR. However, mRNA expression was several orders of magnitude lower in the GI tract compared to placenta. Our results identify a non-placental site of PSG expression in the gut and associated tumors, with implications for determining whether PSGs have a role in tumor progression, and utility as tumor biomarkers. en
dc.description.sponsorship Science Foundation Ireland (Principal Investigator award) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Taylor & Francis en
dc.relation.uri https://www.tandfonline.com/doi/full/10.1080/19420862.2015.1134410
dc.rights © 2016 Taylor & Francis Group, LLC. This is an Accepted Manuscript of an article published by Taylor & Francis in mAbs on 18 March 2016, available online: http://www.tandfonline.com/10.1080/19420862.2015.1134410 en
dc.subject Carcinoembryonic antigen en
dc.subject CEACAM en
dc.subject Colon en
dc.subject Colonic adenocarcinoma en
dc.subject Monoclonal antibody en
dc.subject Placenta en
dc.subject Pregnancy-specific glycoprotein en
dc.subject PSG1 en
dc.subject Oesphagus Squamous epithelium en
dc.subject Squamous cell carcinoma en
dc.subject Trophoblast en
dc.title Pregnancy-specific glycoprotein expression in normal gastrointestinal tract and in tumors detected with novel monoclonal antibodies. en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Aileen Houston, Medicine Department , University College Cork, Cork, Ireland. +353-21-490-3000 Email: a.houston@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2019-09-02T06:13:54Z
dc.description.version Accepted Version en
dc.internal.rssid 350444688
dc.internal.pmid 26926266
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle mAbs en
dc.internal.copyrightchecked No
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress a.houston@ucc.ie en


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