Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid|liquid interface

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Accepted Version
Date
2019-09-12
Authors
Burgoyne, Edward D.
Stockmann, Talia Jane
Molina-Osorio, Andrés F.
Shanahan, Rachel
McGlacken, Gerard P.
Scanlon, Micheál D.
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American Chemical Society
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Abstract
Opportunistic pathogenic bacteria, such as Pseudomonas aeruginosa, pose a serious risk to patients suffering from a compromised immune system and those patients with cystic fibrosis. Confirming their presence often requires culturing the bacteria which can take days. Herein is proposed a rapid electrochemical detection method based on P. aeruginosa generated small molecules employed for chemical communication – referred to as quorum sensing (QS) molecules – within the biofilm formed by the bacteria; specifically, 4‐hydroxy‐2‐heptylquinoline (HHQ) and 2‐heptyl‐3,4‐dihydroxyquinoline (PQS, Pseudomonas quinolone signal). This method does not depend on the redox activity of the QS molecules. Instead, as a proof-of-concept, electrochemical monitoring was achieved through aqueous alkali metal ion and proton interfacial complexation with organic solubilized HHQ and PQS at an interface between two immiscible electrolyt-ic solutions (ITIES), specifically, between water and 1,2-dichloroethane. The proton:HHQ and proton:PQS binding stoi-chiometry’s were discovered to be 1:3 and 1:2, respectively, which is likely due to the relatively high concentrations of QS molecules employed. Owing to the biphasic nature of the methodology, experimental complications due to the poor solubility of the hydrophobic QS molecules in aqueous media were avoided.
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Keywords
Immune system , Cystic fibrosis , Rapid electrochemical detection , Chemical communication , Quorum sensing molecules , Biofilm
Citation
Burgoyne, E. D., Stockmann, T. J., Molina-Osorio, A. F., Shanahan, R., McGlacken, G. P. and Scanlon, M. D. (2019) 'Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid|liquid interface', Journal of Physical Chemistry C. doi: 10.1021/acs.jpcc.9b08350
Copyright
© 2019, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry C, © American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jpcc.9b08350