Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid|liquid interface

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dc.contributor.author Burgoyne, Edward D.
dc.contributor.author Stockmann, Talia Jane
dc.contributor.author Molina-Osorio, Andrés F.
dc.contributor.author Shanahan, Rachel
dc.contributor.author McGlacken, Gerard P.
dc.contributor.author Scanlon, Micheál D.
dc.date.accessioned 2019-09-18T09:12:18Z
dc.date.available 2019-09-18T09:12:18Z
dc.date.issued 2019-09-12
dc.identifier.citation Burgoyne, E. D., Stockmann, T. J., Molina-Osorio, A. F., Shanahan, R., McGlacken, G. P. and Scanlon, M. D. (2019) 'Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid|liquid interface', Journal of Physical Chemistry C. doi: 10.1021/acs.jpcc.9b08350 en
dc.identifier.startpage 1 en
dc.identifier.endpage 15 en
dc.identifier.issn 1932-7447
dc.identifier.uri http://hdl.handle.net/10468/8559
dc.identifier.doi 10.1021/acs.jpcc.9b08350 en
dc.description.abstract Opportunistic pathogenic bacteria, such as Pseudomonas aeruginosa, pose a serious risk to patients suffering from a compromised immune system and those patients with cystic fibrosis. Confirming their presence often requires culturing the bacteria which can take days. Herein is proposed a rapid electrochemical detection method based on P. aeruginosa generated small molecules employed for chemical communication – referred to as quorum sensing (QS) molecules – within the biofilm formed by the bacteria; specifically, 4‐hydroxy‐2‐heptylquinoline (HHQ) and 2‐heptyl‐3,4‐dihydroxyquinoline (PQS, Pseudomonas quinolone signal). This method does not depend on the redox activity of the QS molecules. Instead, as a proof-of-concept, electrochemical monitoring was achieved through aqueous alkali metal ion and proton interfacial complexation with organic solubilized HHQ and PQS at an interface between two immiscible electrolyt-ic solutions (ITIES), specifically, between water and 1,2-dichloroethane. The proton:HHQ and proton:PQS binding stoi-chiometry’s were discovered to be 1:3 and 1:2, respectively, which is likely due to the relatively high concentrations of QS molecules employed. Owing to the biphasic nature of the methodology, experimental complications due to the poor solubility of the hydrophobic QS molecules in aqueous media were avoided. en
dc.description.sponsorship European Research Council (Grant Agreement no. 716792); Irish Research Council (Government of Ireland Postgraduate Scholarship Award - Grant Number GOIPG/2016/1217); Natural Sciences and Engineering Research Council of Canada (Discovery Grant #2019-06074 and Discovery Launch Supplement) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Chemical Society en
dc.rights © 2019, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry C, © American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jpcc.9b08350 en
dc.subject Immune system en
dc.subject Cystic fibrosis en
dc.subject Rapid electrochemical detection en
dc.subject Chemical communication en
dc.subject Quorum sensing molecules en
dc.subject Biofilm en
dc.title Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid|liquid interface en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Gerard P. McGlacken, Chemistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: g.mcglacken@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2020-09-12
dc.date.updated 2019-09-18T08:55:20Z
dc.description.version Accepted Version en
dc.internal.rssid 499775274
dc.contributor.funder European Research Council en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Irish Research Council en
dc.contributor.funder Natural Sciences and Engineering Research Council of Canada en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Physical Chemistry C en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress g.mcglacken@ucc.ie en
dc.internal.bibliocheck Just Accepted. Check vol / issue / page range. Amend citation as necessary. Upload Supplementary Information. en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/13/SIRG/2137/IE/Designing Reactive Functionalised Soft Interfaces _ Self-healing soft materials for solar energy conversion, energy storage, and sustainable low cost hydrogen production/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12/IP/1315/IE/The Direct Arylation of Pyrones, Coumarins, Pyridones and Quinolones/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/12/TIDA/B2405/IE/Optimised detection of key biomarkers of Pseudomonas aeruginosa towards a clinical application/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/ en
dc.identifier.eissn 1932-7455

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