Modulation of antibiotic sensitivity and biofilm formation in Pseudomonas aeruginosa by interspecies signal analogues

The CORA service is operating as normal. For general information on remote access to UCC Library services and collections during the University closure, please visit the main library website at https://libguides.ucc.ie

Show simple item record

dc.contributor.author An, Shi-qi
dc.contributor.author Murtagh, Julie
dc.contributor.author Twomey, Kate B.
dc.contributor.author Gupta, Manoj K.
dc.contributor.author O'Sullivan, Timothy P.
dc.contributor.author Ingram, Rebecca
dc.contributor.author Valvano, Miguel A.
dc.contributor.author Tang, Ji-liang
dc.date.accessioned 2019-09-25T08:42:44Z
dc.date.available 2019-09-25T08:42:44Z
dc.date.issued 2019-05-27
dc.identifier.citation An, S.-q., Murtagh, J., Twomey, K. B., Gupta, M. K., O'Sullivan, T. P., Ingram, R., Valvano, M. A. and Tang, J. L. (2019) 'Modulation of antibiotic sensitivity and biofilm formation in Pseudomonas aeruginosa by interspecies signal analogues', Nature Communications, 10, 2334 (11pp). doi: 10.1038/s41467-019-10271-4 en
dc.identifier.volume 10 en
dc.identifier.startpage 1 en
dc.identifier.endpage 11 en
dc.identifier.issn 2041-1723
dc.identifier.uri http://hdl.handle.net/10468/8613
dc.identifier.doi 10.1038/s41467-019-10271-4 en
dc.description.abstract Pseudomonas aeruginosa, a significant opportunistic pathogen, can participate in inter-species communication through signaling by cis-2-unsaturated fatty acids of the diffusible signal factor (DSF) family. Sensing these signals leads to altered biofilm formation and increased tolerance to various antibiotics, and requires the histidine kinase PA1396. Here, we show that the membrane-associated sensory input domain of PA1396 has five transmembrane helices, two of which are required for DSF sensing. DSF binding is associated with enhanced auto-phosphorylation of PA1396 incorporated into liposomes. Further, we examined the ability of synthetic DSF analogues to modulate or inhibit PA1396 activity. Several of these analogues block the ability of DSF to trigger auto-phosphorylation and gene expression, whereas others act as inverse agonists reducing biofilm formation and antibiotic tolerance, both in vitro and in murine infection models. These analogues may thus represent lead compounds to develop novel adjuvants improving the efficacy of existing antibiotics. en
dc.description.sponsorship Wellcome Trust (Grant 100204/A/12/Z); Education Department of Guangxi Zhuang Autonomous Region (Ba Gui Scholar Program 2014A002); Biotechnology and Biological Sciences Research Council (Grant BB/R012415/1) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Springer Nature Limited en
dc.rights © 2019, the Authors. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third-party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Structure prediction en
dc.subject Membrane-proteins en
dc.subject Sensor kinase en
dc.subject Bacterial en
dc.subject Virulence en
dc.subject Genes en
dc.subject Reconstitution en
dc.subject Tobramycin en
dc.subject Topology en
dc.subject Molecule en
dc.title Modulation of antibiotic sensitivity and biofilm formation in Pseudomonas aeruginosa by interspecies signal analogues en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Tim O'Sullivan, Pharmacy, University College Cork, Cork, Ireland. +353-21-490-3000 Email: tim.osullivan@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2019-09-19T16:59:48Z
dc.description.version Published Version en
dc.internal.rssid 488372063
dc.internal.wokid WOS:000469014400006
dc.contributor.funder Wellcome Trust en
dc.contributor.funder Education Department of Guangxi Zhuang Autonomous Region en
dc.contributor.funder Biotechnology and Biological Sciences Research Council en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Nature Communications en
dc.internal.copyrightchecked No
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress tim.osullivan@ucc.ie en
dc.identifier.articleid 2334 en


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2019, the Authors. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third-party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ Except where otherwise noted, this item's license is described as © 2019, the Authors. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third-party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement