A dairy-derived ghrelinergic hydrolysate modulates food intake in vivo

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dc.contributor.author Howick, Ken
dc.contributor.author Wallace-Fitzsimons, Shauna E.
dc.contributor.author Kandil, Dalia
dc.contributor.author Chruścicka, Barbara
dc.contributor.author Calis, Mert
dc.contributor.author Murphy, Eoin
dc.contributor.author Murray, Brian A.
dc.contributor.author Fernandez, Ayoa
dc.contributor.author Barry, Kate M.
dc.contributor.author Kelly, Phil M.
dc.contributor.author Ryan, Aoife M.
dc.contributor.author Cryan, John F.
dc.contributor.author Griffin, Brendan T.
dc.contributor.author Schellekens, Harriët
dc.date.accessioned 2019-10-02T04:54:38Z
dc.date.available 2019-10-02T04:54:38Z
dc.date.issued 2018-09-15
dc.identifier.citation Howick, K., Wallace-Fitzsimons, S., Kandil, D., Chruścicka, B., Calis, M., Murphy, E., Murray, B., Fernandez, A., Barry, K., Kelly, P. and Ryan, A., 2018. A Dairy-Derived Ghrelinergic Hydrolysate Modulates Food Intake In Vivo. International journal of molecular sciences, 19(9), 2780 (21pp). DOI:10.3390/ijms19092780 en
dc.identifier.volume 19 en
dc.identifier.issued 9 en
dc.identifier.startpage 1 en
dc.identifier.endpage 21 en
dc.identifier.issn 1661-6596
dc.identifier.uri http://hdl.handle.net/10468/8672
dc.identifier.doi 10.3390/ijms19092780 en
dc.description.abstract Recent times have seen an increasing move towards harnessing the health-promoting benefits of food and dietary constituents while providing scientific evidence to substantiate their claims. In particular, the potential for bioactive protein hydrolysates and peptides to enhance health in conjunction with conventional pharmaceutical therapy is being investigated. Dairy-derived proteins have been shown to contain bioactive peptide sequences with various purported health benefits, with effects ranging from the digestive system to cardiovascular circulation, the immune system and the central nervous system. Interestingly, the ability of dairy proteins to modulate metabolism and appetite has recently been reported. The ghrelin receptor (GHSR-1a) is a G-protein coupled receptor which plays a key role in the regulation of food intake. Pharmacological manipulation of the growth hormone secretagogue receptor-type 1a (GHSR-1a) receptor has therefore received a lot of attention as a strategy to combat disorders of appetite and body weight, including age-related malnutrition and the progressive muscle wasting syndrome known as cachexia. In this study, a milk protein-derivative is shown to increase GHSR-1a-mediated intracellular calcium signalling in a concentration-dependent manner in vitro. Significant increases in calcium mobilisation were also observed in a cultured neuronal cell line heterologously expressing the GHS-R1a. In addition, both additive and synergistic effects were observed following co-exposure of GHSR-1a to both the hydrolysate and ghrelin. Subsequent in vivo studies monitored standard chow intake in healthy male and female Sprague-Dawley rats after dosing with the casein hydrolysate (CasHyd). Furthermore, the provision of gastro-protected oral delivery to the bioactive in vivo may aid in the progression of in vitro efficacy to in vivo functionality. In summary, this study reports a ghrelin-stimulating bioactive peptide mixture (CasHyd) with potent effects in vitro. It also provides novel and valuable translational data supporting the potential role of CasHyd as an appetite-enhancing bioactive. Further mechanistic studies are required in order to confirm efficacy as a ghrelinergic bioactive in susceptible population groups. en
dc.description.sponsorship Enterprise Ireland (TC20130001) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher MDPI AG en
dc.relation.uri https://www.mdpi.com/1422-0067/19/9/2780
dc.rights © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Ghrelin en
dc.subject Ghrelin receptor en
dc.subject Bioactive peptides en
dc.subject Dairy en
dc.subject Food intake en
dc.subject Appetite en
dc.subject Calcium mobilisation en
dc.subject Cachexia en
dc.subject Malnutrition en
dc.title A dairy-derived ghrelinergic hydrolysate modulates food intake in vivo en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Harriët Schellekens, Department of Anatomy and Neuroscience, University College Cork, Cork, Ireland. +353-21-490-3000 Email: h.schellekens@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Enterprise Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle International Journal of Molecular Sciences en
dc.internal.IRISemailaddress h.schellekens@ucc.ie en
dc.identifier.articleid 2780 en
dc.identifier.eissn 1422-0067


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© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Except where otherwise noted, this item's license is described as © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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