In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue

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dc.contributor.author Chisholm, K. I.
dc.contributor.author Ida, K. K.
dc.contributor.author Davies, A. L.
dc.contributor.author Papkovsky, Dmitri B.
dc.contributor.author Singer, M.
dc.contributor.author Dyson, A.
dc.contributor.author Tachtsidis, I.
dc.contributor.author Duchen, M. R.
dc.contributor.author Smith, K. J.
dc.date.accessioned 2019-10-25T09:05:34Z
dc.date.available 2019-10-25T09:05:34Z
dc.date.issued 2016-01
dc.identifier.citation Chisholm, K. I., Ida, K. K., Davies, A. L., Papkovsky, D. B., Singer, M., Dyson, A., Tachtsidis, I., Duchen, M. R. and Smith, K. J. (2016) 'In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue', in Elwell, C. E., Leung, T. S. and Harrison, D. K. (eds) Oxygen Transport to Tissue XXXVII. Advances in Experimental Medicine and Biology, vol 876. Springer, New York, NY, pp. 233-239. doi: 10.1007/978-1-4939-3023-4_29 en
dc.identifier.volume 876 en
dc.identifier.startpage 233 en
dc.identifier.endpage 239 en
dc.identifier.isbn 978-1-4939-3022-7
dc.identifier.isbn 978-1-4939-3023-4
dc.identifier.uri http://hdl.handle.net/10468/8857
dc.identifier.doi 10.1007/978-1-4939-3023-4_29 en
dc.description.abstract Live imaging of mitochondrial function is crucial to understand the important role played by these organelles in a wide range of diseases. The mitochondrial redox potential is a particularly informative measure of mitochondrial function, and can be monitored using the endogenous green fluorescence of oxidized mitochondrial flavoproteins. Here, we have observed flavoprotein fluorescence in the exposed murine cerebral cortex in vivo using confocal imaging; the mitochondrial origin of the signal was confirmed using agents known to manipulate mitochondrial redox potential. The effects of cerebral oxygenation on flavoprotein fluorescence were determined by manipulating the inspired oxygen concentration. We report that flavoprotein fluorescence is sensitive to reductions in cortical oxygenation, such that reductions in inspired oxygen resulted in loss of flavoprotein fluorescence with the exception of a preserved ‘halo’ of signal in periarterial regions. The findings are consistent with reports that arteries play an important role in supplying oxygen directly to tissue in the cerebral cortex, maintaining mitochondrial function. en
dc.description.sponsorship University College London (Grand Challenges) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Springer Nature Switzerland AG en
dc.relation.ispartof Advances in Experimental Medicine and Biology book series (AEMB, volume 876)
dc.relation.uri http://www.isott.org/index.php/news-reader/items/isott-2014-london-uk.html
dc.relation.uri https://doi.org/10.1007/978-1-4939-3023-4_66
dc.rights © 2016, the Authors. This chapter was originally published under a CC BY-NC 4.0 license, but has now been made available under a CC BY 4.0 license. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Brain cortex en
dc.subject Mitochondria en
dc.subject Capillaries en
dc.subject Tension en
dc.subject Pressure en
dc.subject Venules en
dc.subject Surface en
dc.subject Disease en
dc.subject Oxygen en
dc.subject Brain en
dc.subject Vasculature en
dc.subject Confocal microscope en
dc.title In vivo imaging of flavoprotein fluorescence during hypoxia reveals the importance of direct arterial oxygen supply to cerebral cortex tissue en
dc.type Conference item en
dc.internal.authorcontactother Dmitri Papkovsky, Biochemistry & Cell Biology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.papkovsky@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2019-10-25T08:37:35Z
dc.description.version Published Version en
dc.internal.rssid 421661438
dc.internal.wokid WOS:000373880600030
dc.contributor.funder Wellcome Trust en
dc.contributor.funder University College London en
dc.contributor.funder Multiple Sclerosis Society en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Advances in Experimental Medicine and Biology en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.conferencelocation London en
dc.internal.IRISemailaddress d.papkovsky@ucc.ie en


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© 2016, the Authors. This chapter was originally published under a CC BY-NC 4.0 license, but has now been made available under a CC BY 4.0 license. Except where otherwise noted, this item's license is described as © 2016, the Authors. This chapter was originally published under a CC BY-NC 4.0 license, but has now been made available under a CC BY 4.0 license.
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