The Lactococcus lactis pan-plasmidome

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dc.contributor.author Kelleher, Philip
dc.contributor.author Mahony, Jennifer
dc.contributor.author Bottacini, Francesca
dc.contributor.author Lugli, Gabriele A.
dc.contributor.author Ventura, Marco
dc.contributor.author van Sinderen, Douwe
dc.date.accessioned 2019-11-19T10:14:21Z
dc.date.available 2019-11-19T10:14:21Z
dc.date.issued 2019-04-04
dc.identifier.citation Kelleher, P., Mahony, J., Bottacini, F., Lugli, G.A., Ventura, M. and Van Sinderen, D., 2019. The Lactococcus lactis pan-plasmidome. Frontiers in microbiology, 10. (19pp). DOI:10.3389/fmicb.2019.00707 en
dc.identifier.volume 10 en
dc.identifier.startpage 1 en
dc.identifier.endpage 20 en
dc.identifier.uri http://hdl.handle.net/10468/9048
dc.identifier.doi 10.3389/fmicb.2019.00707 en
dc.description.abstract Plasmids are autonomous, self-replicating, extrachromosomal genetic elements that are typically not essential for growth of their host. They may encode metabolic capabilities, which promote the maintenance of these genetic elements, and may allow adaption to specific ecological niches and consequently enhance survival. Genome sequencing of 16 Lactococcus lactis strains revealed the presence of 83 plasmids, including two megaplasmids. The limitations of Pacific Biosciences SMRT sequencing in detecting the total plasmid complement of lactococcal strains is examined, while a combined Illumina/SMRT sequencing approach is proposed to combat these issues. Comparative genome analysis of these plasmid sequences combined with other publicly available plasmid sequence data allowed the definition of the lactococcal plasmidome, and facilitated an investigation into (bio)technologically important plasmid-encoded traits such as conjugation, bacteriocin production, exopolysaccharide (EPS) production and (bacterio)phage resistance. en
dc.description.sponsorship CheeseBoard 2015 project (10/RD/TMFRC/704) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fmicb.2019.00707
dc.rights © 2019 Kelleher, Mahony, Bottacini, Lugli, Ventura and van Sinderen en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Lactococcal en
dc.subject Plasmid en
dc.subject SMRT sequencing en
dc.subject Dairy fermentation en
dc.subject Conjugation en
dc.subject Lactic acid bacteria en
dc.title The Lactococcus lactis pan-plasmidome en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe van Sinderen, School of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Fronti Miers incrobiology en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.identifier.articleid 707 en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/ en
dc.identifier.eissn 1664-302X


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© 2019 Kelleher, Mahony, Bottacini, Lugli, Ventura and van Sinderen Except where otherwise noted, this item's license is described as © 2019 Kelleher, Mahony, Bottacini, Lugli, Ventura and van Sinderen
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