Serendipitous identification of natural intergenotypic recombinants of hepatitis C in Ireland

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dc.contributor.author Moreau, Isabelle
dc.contributor.author Hegarty, Susan
dc.contributor.author Levis, John
dc.contributor.author Sheehy, Patrick
dc.contributor.author Crosbie, Orla
dc.contributor.author Kenny-Walsh, Elizabeth
dc.contributor.author Fanning, Liam J.
dc.date.accessioned 2013-01-22T13:37:07Z
dc.date.available 2013-01-22T13:37:07Z
dc.date.copyright 2006
dc.date.issued 2006-06
dc.identifier.citation Moreau, I., Hegarty, S., Levis, J., Sheehy, P., Crosbie, O., Kenny-Walsh, E.,Fanning, L.J. (2006) 'Serendipitous identification of natural Intergenotypic recombinants of hepatitis C in Ireland'. Virology Journal, 3: 95. http://dx.doi.org/10.1186/1743-422X-3-95 en
dc.identifier.volume 3 en
dc.identifier.issued 95 en
dc.identifier.issn 1743-422X
dc.identifier.uri http://hdl.handle.net/10468/909
dc.identifier.doi 10.1186/1743-422X-3-95
dc.description.abstract Background: Recombination between hepatitis C single stranded RNA viruses is a rare event. Natural viable intragenotypic and intergenotypic recombinants between 1b-1a, 1a-1c and 2k-1b, 2i-6p, respectively, have been reported. Diagnostically recombinants represent an intriguing challenge. Hepatitis C genotype is defined by interrogation of the sequence composition of the 5' untranslated region [5' UTR]. Occasionally, ambiguous specimens require further investigation of the genome, usually by interrogation of the NS5B region. The original purpose of this study was to confirm the existence of a suspected mixed genotype infection of genotypes 2 and 4 by clonal analysis at the NS5B region of the genome in two specimens from two separate individuals. This initial identification of genotype was based on analysis of the 5' UTR of the genome by reverse line probe hybridisation [RLPH].Results: The original diagnosis of a mixed genotype infection was not confirmed by clonal analysis of the NS5B region of the genome. The phylogenetic analysis indicated that both specimens were natural intergenotypic recombinant forms of HCV. The recombination was between genotypes 2k and 1b for both specimens. The recombination break point was identified as occurring within the NS2 region of the genome. Conclusion: The viral recombinants identified here resemble the recombinant form originally identified in Russia. The RLPH pattern observed in this study may be a signature indicative of this particular type of intergenotype recombinant of hepatitis C meriting clonal analysis of NS2. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central en
dc.relation.uri http://www.virologyj.com/content/3/1/95
dc.rights © 2006 Moreau et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://creativecommons.org/licenses/by/2.0 en
dc.subject St Petersburg en
dc.subject Virus en
dc.subject Sequence en
dc.subject Infection en
dc.subject Genotype en
dc.subject Patterns en
dc.subject Subtypes en
dc.subject Region en
dc.subject France en
dc.subject Ireland en
dc.subject.lcsh Hepatitis C--Ireland en
dc.title Serendipitous identification of natural intergenotypic recombinants of hepatitis C in Ireland en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://www.ucc.ie/ga/meddept/Staff/DrLiamFanning/ en
dc.internal.authorcontactother Liam Fanning, Medicine Department, University College Cork, Cork, Ireland. +353-21-490-3000 Email: l.fanning@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2013-01-18T15:09:22Z
dc.description.version Published Version en
dc.internal.rssid 160752915
dc.internal.wokid 000247510800001
dc.description.status Peer reviewed en
dc.identifier.journaltitle Virology Journal en
dc.internal.copyrightchecked No - CORA. ROMEO Biomedcentral. Published Version permitted. OA journal en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress l.fanning@ucc.ie en


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© 2006 Moreau et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © 2006 Moreau et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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