Assessment of RNAlater® as a potential method to preserve bovine muscle proteins compared with dry Ice in a proteomic study
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Published version
Published version
Date
2019-02-05
Authors
Zhu, Yao
Mullen, Anne Maria
Rai, Dilip K.
Kelly, Alan L.
Sheehan, David
Cafferky, Jamie
Hamill, Ruth M.
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Published Version
Abstract
RNAlater® is regarded as a potential preservation method for proteins, while its effect on bovine muscle proteins has rarely been evaluated. Bovine muscle protein samples (n = 12) collected from three tender (Warner–Bratzler shear force: 30.02–31.74 N) and three tough (Warner–Bratzler shear force: 54.12–66.25 N) Longissimus thoracis et lumborum (LTL) samples, preserved using two different sampling preservation methods (RNAlater® and dry ice), at two post mortem time points (day 0 and day 14), were characterized using one-dimensional electrophoresis. Fourteen bands with molecular weights ranging from 15 to 250 kDa were verified, both in the dry ice and RNAlater® storage groups, at each time point, using image analysis. A shift from high to low molecular weight fragments, between day 0 and day 14, indicated proteolysis of the muscle proteins during post mortem storage. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses and database searching resulted in the identification of 10 proteins in four bands. Protein profiles of muscle preserved in RNAlater® were similar to those of muscle frozen on dry ice storage, both at day 0 and day 14. The results demonstrate that RNAlater® could be a simple and efficient way to preserve bovine muscle proteins for bovine muscle proteomic studies.
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Keywords
Muscle proteins , One-dimensional electrophoresis , Bovine proteomics , LC-MS/MS , Sample preparation
Citation
Zhu, Y., Mullen, A.M., Rai, D.K., Kelly, A.L., Sheehan, D., Cafferky, J. and Hamill, R.M., 2019. Assessment of RNAlater® as a Potential Method to Preserve Bovine Muscle Proteins Compared with Dry Ice in a Proteomic Study. Foods, 8(2), (60). DOI:10.3390/foods8020060