Separation of Hepatitis C genotype 4a into IgG-depleted and IgG-enriched fractions reveals a unique quasispecies profile

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dc.contributor.author Moreau, Isabelle
dc.contributor.author O'Sullivan, Hilary
dc.contributor.author Murray, Caroline
dc.contributor.author Levis, John
dc.contributor.author Crosbie, Orla
dc.contributor.author Kenny-Walsh, Elizabeth
dc.contributor.author Fanning, Liam J.
dc.date.accessioned 2013-01-26T17:00:36Z
dc.date.available 2013-01-26T17:00:36Z
dc.date.copyright 2008
dc.date.issued 2008-08
dc.identifier.citation Moreau, I, O'Sullivan, H, Murray, C, Levis, J, Crosbie, O, Kenny-Walsh, E, Fanning, LJ (2008) 'Separation of Hepatitis C genotype 4a into IgG-depleted and IgG-enriched fractions reveals a unique quasispecies profile'. Virology Journal, 5:103. doi: 10.1186/1743-422X-5-103 en
dc.identifier.volume 5 en
dc.identifier.issn 1743-422X
dc.identifier.uri http://hdl.handle.net/10468/928
dc.identifier.doi 10.1186/1743-422X-5-103
dc.description.abstract Background: Hepatitis C virus (HCV) circulates in an infected individual as a heterogeneous mixture of closely related viruses called quasispecies. The E1/E2 region of the HCV genome is hypervariable (HVR1) and is targeted by the humoral immune system. Hepatitis C virions are found in two forms: antibody associated or antibody free. The objective of this study was to investigate if separation of Hepatitis C virions into antibody enriched and antibody depleted fractions segregates quasispecies populations into distinctive swarms. Results: A HCV genotype 4a specimen was fractionated into IgG-depleted and IgG-enriched fractions by use of Albumin/IgG depletion spin column. Clonal analysis of these two fractions was performed and then compared to an unfractionated sample. Following sequence analysis it was evident that the antibody depleted fraction was significantly more heterogeneous than the antibody enriched fraction, revealing a unique quasispecies profile. An in-frame 3 nt insertion was observed in 26% of clones in the unfractionated population and in 64% of clones in the IgG-depleted fraction. In addition, an in-frame 3 nt indel event was observed in 10% of clones in the unfractionated population and in 9% of clones in the IgG-depleted fraction. Neither of these latter events, which are rare occurrences in genotype 4a, was identified in the IgG-enriched fraction.Conclusion: In conclusion, the homogeneity of the IgG-enriched species is postulated to represent a sequence that was strongly recognised by the humoral immune system at the time the sample was obtained. The heterogeneous nature of the IgG-depleted fraction is discussed in the context of humoral escape. en
dc.description.sponsorship Health Research Board (505-005-711-4912) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher BioMed Central en
dc.relation.uri http://www.virologyj.com/content/5/1/103
dc.rights © 2008 Moreau et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.rights.uri http://creativecommons.org/licenses/by/2.0/ en
dc.subject Hypervariable region-1 en
dc.subject Neutralising antibodies en
dc.subject Antiviral therapy en
dc.subject Low-density en
dc.subject Life-cycle en
dc.subject Virus en
dc.subject Infection en
dc.subject Recombinant en
dc.subject Enhancement en
dc.subject Particles en
dc.subject.lcsh Hepatitis C virus en
dc.subject.lcsh Immune system en
dc.subject.lcsh Antibodies en
dc.title Separation of Hepatitis C genotype 4a into IgG-depleted and IgG-enriched fractions reveals a unique quasispecies profile en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://www.ucc.ie/ga/meddept/Staff/DrLiamFanning/ en
dc.internal.authorcontactother Liam Fanning, Medicine Department, University College Cork, Cork, Ireland. +353-21-490-3000 Email: l.fanning@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2013-01-18T10:50:14Z
dc.description.version Published Version en
dc.internal.rssid 160751806
dc.internal.wokid 000260031000001
dc.contributor.funder Health Research Board en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Virology Journal en
dc.internal.copyrightchecked No - Publisher's version/PDF may be used. CORA - ROMEO PV permitted. en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress l.fanning@ucc.ie en


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© 2008 Moreau et al; licensee BioMed Central Ltd.  This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Except where otherwise noted, this item's license is described as © 2008 Moreau et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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