Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal

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Date
2019-10-28
Authors
James, Kieran
Bottacini, Francesca
Contreras, Jose Ivan Serrano
Vigoureux, Mariane
Egan, Muireann
Motherway, Mary O’Connell
Holmes, Elaine
van Sinderen, Douwe
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Springer Nature
Published Version
10.1038/s41598-019-51901-7
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Abstract
A number of bifidobacterial species are found at a particularly high prevalence and abundance in faecal samples of healthy breastfed infants, a phenomenon that is believed to be, at least partially, due to the ability of bifidobacteria to metabolize Human Milk Oligosaccharides (HMOs). In the current study, we isolated a novel strain of Bifidobacterium kashiwanohense, named APCKJ1, from the faeces of a four-week old breastfed infant, based on the ability of the strain to utilise the HMO component fucosyllactose. We then determined the full genome sequence of this strain, and employed the generated data to analyze fucosyllactose metabolism in B. kashiwanohense APCKJ1. Transcriptomic and growth analyses, combined with metabolite analysis, in vitro hydrolysis assays and heterologous expression, allowed us to elucidate the pathway for fucosyllactose metabolism in B. kashiwanohense APCKJ1. Homologs of the key genes for this metabolic pathway were identified in particular in infant-derived members of the Bifdobacterium genus, revealing the apparent niche-specific nature of this pathway, and allowing a broad perspective on bifidobacterial fucosyllactose and L-fucose metabolism.
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Keywords
Bacterial genomics , Cellular microbiology , Metabolism
Citation
James, K., Bottacini, F., Contreras, J. I. S., Vigoureux, M., Egan, M., Motherway, M. O. c., Holmes, E. and van Sinderen, D. (2019) 'Metabolism of the predominant human milk oligosaccharide fucosyllactose by an infant gut commensal', Scientific Reports, 9(1), 15427. (20pp.) doi: 10.1038/s41598-019-51901-7
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https://www.nature.com/articles/s41598-020-73762-1
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https://hdl.handle.net/10468/9313
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APC Microbiome Ireland - Journal Articles
Microbiology - Journal Articles
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© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.