Dendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cells

Rahme, Kamil; Minassian, G.; Ghanem, Esther; Souaid, Eddy; Guo, Jianfeng; O'Driscoll, Caitríona M.; Holmes, Justin D.

Dendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cells

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Published version

Date

2018-05

Authors

Rahme, Kamil

Minassian, G.

Ghanem, Esther

Souaid, Eddy

Guo, Jianfeng

O'Driscoll, Caitríona M.

Holmes, Justin D.

Publisher

TechConnect

Abstract

Herein we describe a simple method for the synthesis of dendrigraft poly-L-lysine (d-PLL) coated different sizes gold nanoparticles in water (AuNPs-d-PLL) as potential to delivery vehicles of siRNA to PC-3 prostate cancer cells. AuNPs-d-PLL with diameters ranging between 50-120 nm have been synthesised in aqueous solutions using d-PLL (7 KD) as a capping ligand, and ascorbic acid as a reducing agent. The size of the resulting AuNPs was found to depend on several parameters (i.e. the concentrations of the gold salt, ascorbic d-PLL and temperature). The obtained AuNPs-d-PLL were characterized using UV-visible spectroscopy (UV-vis), Scanning Electron Microscopy (SEM), and Dynamic Light Scattering (DLS). The ability to PEGylate the AuNPs-d-PLL with SH-PEG-OCH3 and SH-PEG-Folate was demonstrated via DLS and zeta potential mesurements, their capacity to complex siRNA was verified (DLS, gel electrophoresis), and transfer of AuNPs-d-PLL-PEG-FA.siRNA to PC-3 cells was investigated.

Keywords

Cancer , Gold nanoparticles , Polymers , siRNA delivery , Stabilization , Phase locked loops , Amino acids , Ascorbic acid , Cells , Diseases , Dynamic light scattering , Electrophoresis , Fiber optic sensors , Metal nanoparticles , RNA , Scanning electron microscopy , Solutions , Stabilization , Synthesis (chemical) , Ultraviolet visible spectroscopy , Urology

Citation

Rahme, K., Minassian, G., Ghanem, E., Souaid, E., Guo, J., O'Driscoll, C. M., and Holmes, J. D. (2018) 'Dendrigraft poly-L-lysine (d-PLL) coated gold nanoparticles in water for siRNA delivery to prostate cancer cells', 11th Annual TechConnect World Innovation Conference and Expo, Anaheim, CA. USA, 13-16 May, TechConnect Briefs, pp. 91-94. isbn: 978-0-9975117-8-9