Uncovering bifidobacteria via targeted sequencing of the mammalian gut microbiota

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dc.contributor.author Lugli, Gabriele Andrea
dc.contributor.author Duranti, Sabrina
dc.contributor.author Milani, Christian
dc.contributor.author Mancabelli, Leonardo
dc.contributor.author Turroni, Francesca
dc.contributor.author van Sinderen, Douwe
dc.contributor.author Ventura, Marco
dc.date.accessioned 2019-12-09T12:37:13Z
dc.date.available 2019-12-09T12:37:13Z
dc.date.issued 2019-11-06
dc.identifier.citation Lugli, G. A., Duranti, S., Milani, C., Mancabelli, L., Turroni, F., van Sinderen, D. and Ventura, M. (2019) 'Uncovering Bifidobacteria via Targeted Sequencing of the Mammalian Gut Microbiota', Microorganisms, 7(11), 535 (11pp). doi: 10.3390/microorganisms7110535 en
dc.identifier.volume 7 en
dc.identifier.issued 11 en
dc.identifier.startpage 1 en
dc.identifier.endpage 11 en
dc.identifier.uri http://hdl.handle.net/10468/9365
dc.identifier.doi 10.3390/microorganisms7110535 en
dc.description.abstract Bifidobacteria are among the most prevalent gut commensals in mammals, playing crucial functional roles that start from their early colonization of the infant gastrointestinal tract and last throughout the life span of their host. Metagenomic approaches have been employed to unveil the genetic features of bifidobacteria in order to understand how they participate in the correct development of a healthy microbiome. Nevertheless, their low relative abundance in many environmental samples may represent a major limitation for metagenomics approaches. To overcome this restriction, we applied an enrichment method that allows amplification of bifidobacterial DNA obtained from human or animal fecal samples for up to 26,500-fold, resulting in the metagenomic reconstruction of genomes belonging to bifidobacterial strains, present at very low abundance in collected samples. Functional predictions of the genes from these reconstructed genomes allows us to identify unique signatures among members of the same bifidobacterial species, highlighting genes correlated with the uptake of nutrients and adhesion to the intestinal mucosa. en
dc.description.sponsorship Science Fondation Ireland (Grant number 15/JP-HDHL/3280, SFI/12/RC/2273a and SFI/12/RC/2273b) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher MDPI AG en
dc.rights © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Genomics en
dc.subject Metagenomics en
dc.subject Microbiota en
dc.subject Bifidobacterium en
dc.title Uncovering bifidobacteria via targeted sequencing of the mammalian gut microbiota en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Douwe van Sinderen, School of Micrcobiology and APC Microbiome Ireland, University College Cork, Cork, Ireland. +353-21-490-3000 Email:d.vansinderen@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder European Commission en
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Microorganisms en
dc.internal.IRISemailaddress d.vansinderen@ucc.ie en
dc.identifier.articleid 535 en
dc.identifier.eissn 2076-2607


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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Except where otherwise noted, this item's license is described as © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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