Metagenomics of Atacama lithobiontic extremophile life unveils highlights on fungal communities, biogeochemical cycles and carbohydrate-active enzymes

Show simple item record

dc.contributor.author Gómez-Silva, Benito
dc.contributor.author Vilo-Muñoz, Claudia
dc.contributor.author Galetović, Alexandra
dc.contributor.author Dong, Qunfeng
dc.contributor.author Castelán-Sánchez, Hugo G.
dc.contributor.author Pérez-Llano, Yordanis
dc.contributor.author Sánchez-Carbente, María del Rayo
dc.contributor.author Dávila-Ramos, Sonia
dc.contributor.author Cortés-López, Nohemí Gabriela
dc.contributor.author Martínez-Ávila, Liliana
dc.contributor.author Dobson, Alan D. W.
dc.contributor.author Batista-García, Ramón Alberto
dc.date.accessioned 2019-12-10T09:20:44Z
dc.date.available 2019-12-10T09:20:44Z
dc.date.issued 2019-11-27
dc.identifier.citation Gómez-Silva, B., Vilo-Muñoz, C., Galetović, A., Dong, Q., Castelán-Sánchez, H. G., Pérez-Llano, Y., Sánchez-Carbente, M. d. R., Dávila-Ramos, S., Cortés-López, N. G., Martínez-Ávila, L., Dobson, A. D. W. and Batista-García, R. A. (2019) 'Metagenomics of Atacama Lithobiontic Extremophile Life Unveils Highlights on Fungal Communities, Biogeochemical Cycles and Carbohydrate-Active Enzymes', Microorganisms, 7(12), 619 (25pp). doi: 10.3390/microorganisms7120619 en
dc.identifier.volume 7 en
dc.identifier.issued 12 en
dc.identifier.startpage 1 en
dc.identifier.endpage 25 en
dc.identifier.uri http://hdl.handle.net/10468/9368
dc.identifier.doi 10.3390/microorganisms7120619 en
dc.description.abstract Halites, which are typically found in various Atacama locations, are evaporitic rocks that are considered as micro-scaled salterns. Both structural and functional metagenomic analyses of halite nodules were performed. Structural analyses indicated that the halite microbiota is mainly composed of NaCl-adapted microorganisms. In addition, halites appear to harbor a limited diversity of fungal families together with a biodiverse collection of protozoa. Functional analysis indicated that the halite microbiome possesses the capacity to make an extensive contribution to carbon, nitrogen, and sulfur cycles, but possess a limited capacity to fix nitrogen. The halite metagenome also contains a vast repertory of carbohydrate active enzymes (CAZY) with glycosyl transferases being the most abundant class present, followed by glycosyl hydrolases (GH). Amylases were also present in high abundance, with GH also being identified. Thus, the halite microbiota is a potential useful source of novel enzymes that could have biotechnological applicability. This is the first metagenomic report of fungi and protozoa as endolithobionts of halite nodules, as well as the first attempt to describe the repertoire of CAZY in this community. In addition, we present a comprehensive functional metagenomic analysis of the metabolic capacities of the halite microbiota, providing evidence for the first time on the sulfur cycle in Atacama halites. en
dc.description.sponsorship CeBiB (FB-0001); Universidad de Antofagasta (SI-5305); UT Health San Antonio (Project Number NIH-NCI P30 CA054174); CPRIT Core Facility (Award Number RP160732); Irish Department of Agriculture, Food and the Marine (DAFM) (SMI-BIO project (15/F/698)); CONACyT-Mexico (Projects No. 285816) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher MDPI en
dc.rights © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Atacame Desert en
dc.subject Halite en
dc.subject Functional metagenomics en
dc.subject Endolithic fungi en
dc.subject CAZyme en
dc.title Metagenomics of Atacama lithobiontic extremophile life unveils highlights on fungal communities, biogeochemical cycles and carbohydrate-active enzymes en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Alan Dobson, School of Microbiology and Environmental Research Institute, University College Cork, Cork, Ireland. +353-21-490-3000 Email:a.dobson@ucc.ie en
dc.internal.availability a.dobson@ucc.ie en
dc.description.version Published Version en
dc.contributor.funder Universidad de Antofagasta en
dc.contributor.funder University of Texas Health Science Center at San Antonio en
dc.contributor.funder National Institutes of Health en
dc.contributor.funder Cancer Prevention and Research Institute of Texas en
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.contributor.funder Consejo Nacional de Ciencia y Tecnología en
dc.contributor.funder Comisión Nacional de Investigación Científica y Tecnológica en
dc.contributor.funder Centre for Biotechnology & Bioinformatics en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Microorganisms en
dc.identifier.articleid 619 en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/299091/EU/How do groundwater-surface water interactions control recharge from ephemeral streams?/EPHEMERAL GSI en
dc.relation.project info:eu-repo/grantAgreement/NIH/OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH/1S10OD021805-01/US/High Throughput DNA Sequencer: Illumina HiSeq 3000 Sequencer/ en
dc.identifier.eissn 2076-2607


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Except where otherwise noted, this item's license is described as © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement