Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators

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Date
2019-11-28
Authors
Lanigan, Noreen
Kelly, Emer
Arzamasov, Aleksandr A.
Stanton, Catherine
Rodionov, Dmitry A.
van Sinderen, Douwe
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Springer Nature
Published Version
10.1038/s41598-019-54229-4
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Abstract
Bifidobacteria resident in the gastrointestinal tract (GIT) are subject to constantly changing environmental conditions, which require rapid adjustments in gene expression. Here, we show that two predicted LacI-type transcription factors (TFs), designated AraQ and MalR1, are involved in regulating the central, carbohydrate-associated metabolic pathway (the so-called phosphoketolase pathway or bifid shunt) of the gut commensal Bifidobacterium breve UCC2003. These TFs appear to not only control transcription of genes involved in the bifid shunt and each other, but also seem to commonly and directly affect transcription of other TF-encoding genes, as well as genes related to uptake and metabolism of various carbohydrates. This complex and interactive network of AraQ/MalR1-mediated gene regulation provides previously unknown insights into the governance of carbon metabolism in bifidobacteria.
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Keywords
Bacterial genetics , Bacterial genomics , Cellular microbiology
Citation
Lanigan, N., Kelly, E., Arzamasov, A. A., Stanton, C., Rodionov, D. A. and van Sinderen, D. (2019) 'Transcriptional control of central carbon metabolic flux in Bifidobacteria by two functionally similar, yet distinct LacI-type regulators', Scientific Reports, 9(1), 17851 (15pp). doi: 10.1038/s41598-019-54229-4
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https://hdl.handle.net/10468/9369
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Microbiology - Journal Articles
APC Microbiome Ireland - Journal Articles
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© 2019, the Authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.