Different carbon sources result in differential activation of sigma B and stress resistance in Listeria monocytogenes

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dc.contributor.author Crespo Tapia, Natalia
dc.contributor.author Dorey, Amber L.
dc.contributor.author Gahan, Cormac G. M.
dc.contributor.author den Besten, Heidy M. W.
dc.contributor.author O'Byrne, Conor P.
dc.contributor.author Abee, Tjakko
dc.date.accessioned 2020-01-10T12:14:30Z
dc.date.available 2020-01-10T12:14:30Z
dc.date.issued 2019-12-30
dc.identifier.citation Crespo Tapia, N., Dorey, A. L., Gahan, C. G. M., den Besten, H. M. W., O'Byrne, C. P. and Abee, T. (2019) 'Different carbon sources result in differential activation of sigma B and stress resistance in Listeria monocytogenes', International Journal of Food Microbiology. doi: 10.1016/j.ijfoodmicro.2019.108504 en
dc.identifier.issn 0168-1605
dc.identifier.uri http://hdl.handle.net/10468/9483
dc.identifier.doi 10.1016/j.ijfoodmicro.2019.108504 en
dc.description.abstract Listeria monocytogenes is an important food-borne pathogen that is ubiquitous in the environment. It is able to utilize a variety of carbon sources, to produce biofilms on food-processing surfaces and to survive food preservation–associated stresses. In this study, we investigated the effect of three common carbon sources, namely glucose, glycerol and lactose, on growth and activation of the general stress response Sigma factor, SigB, and corresponding phenotypes including stress resistance. A fluorescent reporter coupled to the promoter of lmo2230, a highly SigB-dependent gene, was used to determine SigB activation via quantitative fluorescence spectroscopy. This approach, combined with Western blotting and fluorescence microscopy, showed the highest SigB activation in lactose grown cells and lowest in glucose grown cells. In line with this observation, lactose grown cells showed the highest resistance to lethal heat and acid stress, the highest biofilm formation, and had the highest adhesion/invasion capacity in Caco-2-derived C2Bbe1 cell lines. Our data suggest that lactose utilisation triggers a strong SigB dependent stress response and this may have implications for the resistance of L. monocytogenes along the food chain. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier B.V. en
dc.relation.uri http://www.sciencedirect.com/science/article/pii/S0168160519304350
dc.rights © 2019, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license. en
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject Carbon source en
dc.subject Glucose en
dc.subject Glycerol en
dc.subject Lactose en
dc.subject Sigma B en
dc.subject SigB en
dc.subject Stres en
dc.subject Stress response en
dc.subject Heat stress en
dc.subject Acid stress en
dc.subject Biofilm en
dc.subject Virulence en
dc.subject Adhesion assay en
dc.subject Invasion assay en
dc.subject C2Bbe1 cell line en
dc.title Different carbon sources result in differential activation of sigma B and stress resistance in Listeria monocytogenes en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Cormac Gahan, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: c.gahan@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2020-12-30
dc.date.updated 2020-01-07T12:37:09Z
dc.description.version Accepted Version en
dc.internal.rssid 500172918
dc.contributor.funder Horizon 2020 en
dc.description.status Peer reviewed en
dc.identifier.journaltitle International Journal of Food Microbiology en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress c.gahan@ucc.ie en
dc.internal.bibliocheck In press. Check vol / issue / page range. Amend citation as necessary. en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::MSCA-ITN-ETN/641984/EU/Training and research in Listeria monocytogenes Adaptation through Proteomic and Transcriptome deep Sequencing Analysis/List_MAPS en
dc.identifier.eissn 1879-3460


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© 2019, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license. Except where otherwise noted, this item's license is described as © 2019, Elsevier B.V. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.
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