Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, inflammatory cytokine and gut microbiota modulation

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dc.contributor.author Chen, Yang
dc.contributor.author Yang, Bo
dc.contributor.author Ross, R. Paul
dc.contributor.author Jin, Yan
dc.contributor.author Stanton, Catherine
dc.contributor.author Zhao, Jianxin
dc.contributor.author Zhang, Hao
dc.contributor.author Chen, Wei
dc.date.accessioned 2020-01-14T11:00:03Z
dc.date.available 2020-01-14T11:00:03Z
dc.date.issued 2019-11-06
dc.identifier.citation Chen, Y., Yang, B., Ross, R. P., Jin, Y., Stanton, C., Zhao, J., Zhang, H. and Chen, W. (2019) 'Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, inflammatory cytokine and gut microbiota modulation', Journal of Agricultural and Food Chemistry, 67(48), pp. 13282-13298. doi: 10.1021/acs.jafc.9b05744 en
dc.identifier.volume 67 en
dc.identifier.issued 48 en
dc.identifier.startpage 13282 en
dc.identifier.endpage 13298 en
dc.identifier.issn 0021-8561
dc.identifier.uri http://hdl.handle.net/10468/9500
dc.identifier.doi 10.1021/acs.jafc.9b05744 en
dc.description.abstract Dietary supplementation with CLA has been reported to alleviate the effect of colitis in mice, but the mechanisms involved need further exploration. The study aimed to investigate how orally administered CLA alleviates DSS-induced colitis in mice. CLA was administered at five different doses: 40 mg/d, 20 mg/d, 10 mg/d, 5 mg/d and 2.5 mg/d. Doses of CLA at 10 mg/d and higher alleviated colitis symptoms and reduced inflammation induced by DSS, in which 40 mg/d, 20 mg/d and 10 mg/d CLA significantly increased the concentration of MUC2 and goblet cells, but neither 5 mg/d CLA nor 2.5 mg/d CLA had any effects. Meanwhile, 40 mg/d CLA and 20 mg/d CLA treatments significantly up-regulated the concentration of tight junction proteins (ZO-1, occludin and claudin-3) and ameliorated epithelial apoptosis caused by DSS. Moreover, oxidative stress-related enzymes (SOD, GSH-PX, CAT) and inflammatory cytokines (TNF-a, IL-10, IL-6) were modulated by 40 mg/d CLA and 20 mg/d CLA. Furthermore, 40 mg/d CLA rebalanced the gut microbiota damaged by DSS, including reducing Bacteroides and increasing Bifidobacterium and Odoribacter. In conclusion, CLA supplementation alleviated DSS-induced colitis in a dose-dependent manner by modulating inflammatory cytokines and oxidation stress, maintaining the mucosal barrier and reverting microbiota changes. en
dc.description.sponsorship National Natural Science Foundation of China (Nos. 31801521; 31722041); Fundamental Research Funds for the Central Universities (No. JUSRP51702A); National First-Class Discipline Program of Food Science and Technology, China (JUFSTR20180102); Postgraduate Research and Practice Innovation Program of Jiangsu Province, China (KYCX19_1829); Wuxi Young Talent Foundation, China (QNRC075) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher ACS Publications en
dc.rights © 2019, American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, © American Chemical Society, after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.jafc.9b05744 en
dc.subject Conjugated linoleic acid en
dc.subject Colitis en
dc.subject Intestinal barrier function en
dc.subject Oxidative stress en
dc.subject Gut microbiota en
dc.title Orally administered CLA ameliorates DSS-induced colitis in mice via intestinal barrier improvement, oxidative stress reduction, inflammatory cytokine and gut microbiota modulation en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Paul Ross, Alimentary Pharmabotic Centre (APC), University College Cork, Cork, Ireland. +353-21-490-3000 Email: p.ross@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher. en
dc.check.date 2020-11-06
dc.date.updated 2019-11-14T09:38:03Z
dc.description.version Accepted Version en
dc.internal.rssid 499913195
dc.contributor.funder National Natural Science Foundation of China en
dc.contributor.funder Fundamental Research Funds for the Central Universities en
dc.contributor.funder National First-Class Discipline Program of Food Science and Technology, China en
dc.contributor.funder Postgraduate Research and Practice Innovation Program of Jiangsu Province, China en
dc.contributor.funder Wuxi Young Talent Foundation, China en
dc.contributor.funder Jiangsu Province Collaborative Innovation Center for Food Safety and Quality Control, China en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Agricultural and Food Chemistry en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress p.ross@ucc.ie en
dc.identifier.eissn 1520-5118


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