Bacteriophages phi MR299-2 and phi NH-4 can eliminate Pseudomonas aeruginosa in the murine Lung and on Cystic Fibrosis lung airway cells

Show simple item record

dc.contributor.author Alemayehu, Debebe
dc.contributor.author Casey, Pat G.
dc.contributor.author McAuliffe, Olivia
dc.contributor.author Guinane, Caitriona M.
dc.contributor.author Martin, James G.
dc.contributor.author Shanahan, Fergus
dc.contributor.author Coffey, Aidan
dc.contributor.author Ross, R. Paul
dc.contributor.author Hill, Colin
dc.date.accessioned 2013-02-14T16:37:17Z
dc.date.available 2013-02-14T16:37:17Z
dc.date.copyright 2012
dc.date.issued 2012-01
dc.identifier.citation Alemayehu, D; Casey, PG; McAuliffe, O; Guinane, CM; Martin, JG; Shanahan, F; Coffey, A; Ross, RP; Hill, C (2012) 'Bacteriophages phi MR299-2 and phi NH-4 Can Eliminate Pseudomonas aeruginosa in the Murine Lung and on Cystic Fibrosis Lung Airway Cells'. Mbio, 3 . doi: 10.1128/mBio.00029-12 en
dc.identifier.volume 3 en
dc.identifier.issn 2150-7511
dc.identifier.uri http://hdl.handle.net/10468/969
dc.identifier.doi 10.1128/​mBio.00029-12
dc.description.abstract Pseudomonas aeruginosa is a common cause of infection in the lungs of patients with cystic fibrosis (CF). In addition, biofilm formation and antibiotic resistance of Pseudomonas are major problems that can complicate antibiotic therapy. We evaluated the efficacy of using bacteriophages to kill the pathogen in both biofilms and in the murine lung. We isolated and characterized two phages from a local wastewater treatment plant, a myovirus (phi NH-4) and a podovirus (phi MR299-2). Both phages were active against clinical isolates of P. aeruginosa. Together, the two phages killed all 9 clinical isolate strains tested, including both mucoid and nonmucoid strains. An equal mixture of the two phages was effective in killing P. aeruginosa NH57388A (mucoid) and P. aeruginosa MR299 (nonmucoid) strains when growing as a biofilm on a cystic fibrosis bronchial epithelial CFBE41o-cell line. Phage titers increased almost 100-fold over a 24-h period, confirming replication of the phage. Furthermore, the phage mix was also effective in killing the pathogen in murine lungs containing 1 x 10(7) to 2 x 10(7) P. aeruginosa. Pseudomonas was effectively cleared (reduced by a magnitude of at least 3 to 4 log units) from murine lungs in 6 h. Our study demonstrates the efficacy of these two phages in killing clinical Pseudomonas isolates in the murine lung or as a biofilm on a pulmonary cell line and supports the growing interest in using phage therapy for the control and treatment of multidrug-resistant Pseudomonas lung infections in CF patients.IMPORTANCE Given the rise in antibiotic resistance, nonantibiotic therapies are required for the treatment of infection. This is particularly true for the treatment of Pseudomonas infection in patients with cystic fibrosis. We have identified two bacterial viruses (bacteriophages) that can kill Pseudomonas growing on human lung cells and in an animal model of lung infection. The use of bacteriophages is particularly appropriate because the killing agent can replicate on the target cell, generating fresh copies of the bacteriophage. Thus, in the presence of a target, the killing agent multiplies. By using two bacteriophages we can reduce the risk of resistant colonies developing at the site of infection. Bacteriophage therapy is an exciting field, and this study represents an important demonstration of efficacy in validated infection models. en
dc.description.sponsorship Science Foundation Ireland grants 02/CEB124 and 07/CE/B1368. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.relation.uri http://mbio.asm.org/content/3/2/e00029-12.abstract
dc.rights © 2012 Alemayehu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited en
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ en
dc.subject Burkholderia-cepacia en
dc.subject Epithelial-cells en
dc.subject Biofim en
dc.subject Infections en
dc.subject Genome en
dc.subject Gene en
dc.subject Identification en
dc.subject Chromosome en
dc.subject Differentiation en
dc.subject Antibiotics en
dc.subject.lcsh Cystic fibrosis en
dc.title Bacteriophages phi MR299-2 and phi NH-4 can eliminate Pseudomonas aeruginosa in the murine Lung and on Cystic Fibrosis lung airway cells en
dc.type Article (peer-reviewed) en
dc.internal.authorurl http://research.ucc.ie/profiles/D010/chill en
dc.internal.authorcontactother Colin Hill, Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: c.hill@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2013-02-04T12:58:42Z
dc.description.version Published Version en
dc.internal.rssid 160747266
dc.internal.wokid 000305297100007
dc.contributor.funder Science Foundation Ireland en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Mbio en
dc.internal.copyrightchecked No. CORA - SHERPA ROMEO - Published Version permitted en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress c.hill@ucc.ie en


Files in this item

This item appears in the following Collection(s)

Show simple item record

© 2012 Alemayehu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited Except where otherwise noted, this item's license is described as © 2012 Alemayehu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement