Development of multiple strain competitive index assays for Listeria monocytogenes using pIMC; a new site-specific integrative vector

Monk, Ian R.; Casey, Pat G.; Cronin, Michael S.; Gahan, Cormac G.; Hill, Colin

Development of multiple strain competitive index assays for Listeria monocytogenes using pIMC; a new site-specific integrative vector

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Date

2008-06

Authors

Monk, Ian R.

Casey, Pat G.

Cronin, Michael S.

Gahan, Cormac G.

Hill, Colin

Publisher

BioMed Central

Published Version

10.1186/1471-2180-8-96

Abstract

The foodborne, gram-positive pathogen, Listeria monocytogenes, is capable of causing lethal infections in compromised individuals. In the post genomic era of L. monocytogenes research, techniques are required to identify and validate genes involved in the pathogenicity and environmental biology of the organism. The aim here was to develop a widely applicable method to tag L. monocytogenes strains, with a particular emphasis on the development of multiple strain competitive index assays.

Keywords

Pathogen , Infection , Multiple strain competitive index assays , Integrative vector

Citation

Monk IR; Casey PG; Cronin M; Gahan CG; Hill C; (2008) 'Development of multiple strain competitive index assays for Listeria monocytogenes using pIMC; a new site-specific integrative vector'. BMC Microbiology, 8:96.