c9, t11, c15-CLNA and c9, t11, t15-CLNA from Lactobacillus plantarum ZS2058 ameliorate DSS-induced colitis in mice

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dc.contributor.author Ren, Qing
dc.contributor.author Yang, Bo
dc.contributor.author Zhang, Hao
dc.contributor.author Ross, R. Paul
dc.contributor.author Stanton, Catherine
dc.contributor.author Chen, Haiqin
dc.contributor.author Chen, Wei
dc.date.accessioned 2020-03-10T17:02:37Z
dc.date.available 2020-03-10T17:02:37Z
dc.date.issued 2020-03-03
dc.identifier.citation Ren, Q., Yang, B., Zhang, H., Ross, R. P., Stanton, C., Chen, H. and Chen, W. (2020) 'c9, t11, c15-CLNA and c9, t11, t15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate DSS-Induced Colitis in Mice', Journal of Agricultural and Food Chemistry, In Press, doi: 10.1021/acs.jafc.0c00573 en
dc.identifier.startpage 1 en
dc.identifier.endpage 41 en
dc.identifier.issn 0021-8561
dc.identifier.uri http://hdl.handle.net/10468/9746
dc.identifier.doi 10.1021/acs.jafc.0c00573 en
dc.description.abstract The probiotic bacterial strain Lactobacillus plantarum ZS2058 has been proven to alleviate colitis due to its ability to synthesize conjugated fatty acids (CFAs). To investigate the specific functions of CFAs produced by this probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated α-linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and c9, t11, t15-CLNA (CLNA2). The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored. CLNA significantly inhibited weight loss, the disease activity index, colon shortening. Additionally, CLNA improved histological damage, protected colonic mucous layer integrity and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, and claudin-3). CLNA significantly attenuated the expression of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor PPARγ. Moreover, the activity of oxidative stress-related enzymes (superoxide dismutase, glutathione peroxidase and catalase) was increased by CLNA. The myeloperoxidase activity was significantly decreased by CLNA. Meanwhile, the concentrations of CLNA in the liver and colonic conjugated linoleic acid (CLA) were significantly increased in response to CLNA supplementation. Furthermore, CLNA could rebalance the gut microbial composition damaged by DSS, including increasing the α-diversity. CLNA1 and CLNA2 increased the abundance of Ruminococcus and Prevotella, respectively. en
dc.description.sponsorship National Natural Science Foundation of China (No. 486 31722041, 31801521, 31530056); Fundamental Research Funds for the Central Universities (No. JUSRP51702A, JUSRP11733); State Key Laboratory of Food Science and Technology (the national first-class discipline program of Food Science and Technology (JUFSTR20180102)); Jiangsu Province “Collaborative Innovation Center for Food Safety and Quality Control”, the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX18_1763) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Chemical Society en
dc.relation.uri https://pubs.acs.org/doi/10.1021/acs.jafc.0c00573
dc.rights © 2020 American Chemical Society. This document is the Accepted Manuscript version of a Published Work to appear in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jafc.0c00573 en
dc.subject Lactobacillus plantarum ZS2058 en
dc.subject Probiotics en
dc.subject Colitis en
dc.subject Gut en
dc.subject Microbials en
dc.subject Gut microbial composition en
dc.title c9, t11, c15-CLNA and c9, t11, t15-CLNA from Lactobacillus plantarum ZS2058 ameliorate DSS-induced colitis in mice en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Paul Ross, APC Microbiome, University College Cork, Cork, Ireland. +353-21-490-3000 Email: p.ross@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher en
dc.check.date 2021-03-03
dc.date.updated 2020-03-10T16:50:03Z
dc.description.version Accepted Version en
dc.internal.rssid 505705732
dc.contributor.funder National Natural Science Foundation of China en
dc.contributor.funder Fundamental Research Funds for the Central Universities en
dc.contributor.funder State Key Laboratory of Food Science and Technology en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Journal of Agricultural and Food Chemistry en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress p.ross@ucc.ie en
dc.internal.bibliocheck In press. Check vol / issue / page range. Update citation, rights statement en


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