Lutein-mediated photoprotection of photosynthetic machinery in Arabidopsis thaliana exposed to chronic low ultraviolet-B radiation

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dc.contributor.author Biswas, Dilip K.
dc.contributor.author Ma, Bao-Luo
dc.contributor.author Xu, Hong
dc.contributor.author Li, Yonggeng
dc.contributor.author Jiang, Gaoming
dc.date.accessioned 2020-04-14T10:29:37Z
dc.date.available 2020-04-14T10:29:37Z
dc.date.issued 2020-03-30
dc.identifier.citation Biswas, D. K., Ma, B.-L., Xu, H., Li, Y. and Jiang, G. (2020) 'Lutein-mediated photoprotection of photosynthetic machinery in Arabidopsis thaliana exposed to chronic low ultraviolet-B radiation', Plant Physiology, 248, 153160. doi: 10.1016/j.jplph.2020.153160 en
dc.identifier.volume 248 en
dc.identifier.issn 0176-1617
dc.identifier.uri http://hdl.handle.net/10468/9822
dc.identifier.doi 10.1016/j.jplph.2020.153160 en
dc.description.abstract Ecologically relevant low UV-B is reported to alter reactive oxygen species metabolism and anti-oxidative systems through an up-regulation of enzymes of the phenylpropanoid pathway. However, little is known about low UV-B-induced changes in carotenoid profile and their impacts on light harvesting and photoprotection of photosystem II (PSII) in plants. We investigated carotenoids profile, chlorophyll pigments, phenolics, photosynthetic efficiency and growth in Arabidopsis thaliana (Col-0) plants grown under photosynthetically active radiation (PAR), PAR+ ultraviolet (UV)-A and PAR+UV-A+B regimes for 10 days in order to assess plant acclimation to low UV-B radiation. A chlorophyll fluorescence assay was used to examine UV-B tolerance in plants further exposed to acute high UV-B for 4 and 6 h following a 10-day growth under different PAR and UV regimes. We found that both PAR+ UV-A and PAR+UV-A+B regimes had no negative effect on quantum efficiency, electron transport rate, rosette diameter, relative growth rate and shoot dry weight of plants. Chronic PAR+ UV-A regime considerably (P < 0.05) increased violaxanthin (26 %) and neoxanthin (92 %) content in plants. Plant exposure to chronic PAR+UV-A+B significantly (P < 0.05) increased violaxanthin (48 %), neoxanthin (63 %), lutein (33 %), 9-cis ß-carotene (28 %), total ß-carotene (29 %) and total phenolics (108 %). The maximum photochemical efficiency (Fv/Fm) in leaves was found to be positively correlated with total phenolics (rho = 0.81 and rho = 0.91, P < 0.05 for 4 and 6 h, respectively) and non-photochemical quenching (qN) (rho = 0.81 and rho = 0.84, P < 0.05 for 4 and 6 h, respectively) in plants exposed to acute high UV-B for 4 and 6 h following a 10-day growth under chronic PAR+UV-A+B. There was also a significant positive correlation (rho = 0.93, P < 0.01) between qN and lutein content in the plants exposed to acute high UV-B stress for 4 h following plant exposure to chronic PAR+UV-A+B. The findings from our study indicate that plants grown under chronic PAR+UV-A+B displayed higher photoprotection of PSII against acute high UV-B stress than those grown under PAR and PAR+ UV-A regimes. An induction of phenolics and lutein-mediated development of qN were involved in the photoprotection of PSII against UV-B-induced oxidative stress. en
dc.description.sponsorship Irish Research Council for Science, Engineering and Technology (IRCSET EMBARK); Agriculture and Agri-Food Canada and Ottawa Research and Development Centre (Contribution No. 20-xxx) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Elsevier GmbH en
dc.rights © 2020, Elsevier GmbH. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license. en
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/ en
dc.subject Arabidopsis thaliana en
dc.subject Carotenoids en
dc.subject Chl a fluorescence en
dc.subject Lutein en
dc.subject Phenolics en
dc.subject Photoprotection en
dc.subject UV-B acclimation en
dc.subject UV-B tolerance en
dc.title Lutein-mediated photoprotection of photosynthetic machinery in Arabidopsis thaliana exposed to chronic low ultraviolet-B radiation en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Dilip Biswas, Zoology & Ecology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: d.biswas@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 24 months after publication by request of the publisher. en
dc.check.date 2022-03-30
dc.date.updated 2020-04-14T09:41:07Z
dc.description.version Accepted Version en
dc.internal.rssid 510352981
dc.contributor.funder Irish Research Council for Science, Engineering and Technology en
dc.contributor.funder Agriculture and Agri-Food Canada en
dc.contributor.funder Ottawa Research and Development Centre en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Plant Physiology en
dc.internal.copyrightchecked Yes
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress d.biswas@ucc.ie en
dc.internal.bibliocheck Add page range. Amend citation accordingly. en
dc.identifier.eissn 1532-2548


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© 2020, Elsevier GmbH. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license. Except where otherwise noted, this item's license is described as © 2020, Elsevier GmbH. All rights reserved. This manuscript version is made available under the CC BY-NC-ND 4.0 license.
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