Microbiota and metabolite profiling reveal specific alterations in bacterial community structure and environment in the cystic fibrosis airway during exacerbation

dc.contributor.authorTwomey, Kate B.
dc.contributor.authorAlston, Mark
dc.contributor.authorAn, Shi-Qi
dc.contributor.authorO'Connell, Oisin J.
dc.contributor.authorMcCarthy, Yvonne
dc.contributor.authorSwarbreck, David
dc.contributor.authorFebrer, Melanie
dc.contributor.authorDow, J. Maxwell
dc.contributor.authorPlant, Barry J.
dc.contributor.authorRyan, Robert P.
dc.date.accessioned2016-02-17T11:44:41Z
dc.date.available2016-02-17T11:44:41Z
dc.date.issued2013
dc.description.abstractChronic polymicrobial infections of the lung are the foremost cause of morbidity and mortality in cystic fibrosis (CF) patients. The composition of the microbial flora of the airway alters considerably during infection, particularly during patient exacerbation. An understanding of which organisms are growing, their environment and their behaviour in the airway is of importance for designing antibiotic treatment regimes and for patient prognosis. To this end, we have analysed sputum samples taken from separate cohorts of CF and non-CF subjects for metabolites and in parallel, and we have examined both isolated DNA and RNA for the presence of 16S rRNA genes and transcripts by high-throughput sequencing of amplicon or cDNA libraries. This analysis revealed that although the population size of all dominant orders of bacteria as measured by DNA-and RNA-based methods are similar, greater discrepancies are seen with less prevalent organisms, some of which we associated with CF for the first time. Additionally, we identified a strong relationship between the abundance of specific anaerobes and fluctuations in several metabolites including lactate and putrescine during patient exacerbation. This study has hence identified organisms whose occurrence within the CF microbiome has been hitherto unreported and has revealed potential metabolic biomarkers for exacerbation.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide82432
dc.identifier.citationTwomey KB, Alston M, An S-Q, O'Connell OJ, McCarthy Y, Swarbreck D, et al. (2013) Microbiota and Metabolite Profiling Reveal Specific Alterations in Bacterial Community Structure and Environment in the Cystic Fibrosis Airway during Exacerbation. PLoS ONE 8(12): e82432. doi:10.1371/journal.pone.0082432
dc.identifier.doi10.1371/journal.pone.0082432
dc.identifier.issn1932-6203
dc.identifier.issued12en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.urihttps://hdl.handle.net/10468/2348
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2015 Twomey et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBronchoalveolar lavage fluiden
dc.subjectPseudomonas aeruginosaen
dc.subjectLung infectionsen
dc.subjectSputumen
dc.subjectPathogenesisen
dc.subjectBiomarkersen
dc.subjectToleranceen
dc.subjectDiversityen
dc.subjectCellsen
dc.titleMicrobiota and metabolite profiling reveal specific alterations in bacterial community structure and environment in the cystic fibrosis airway during exacerbationen
dc.typeArticle (peer-reviewed)en
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