In vitro characteristics of phages to guide ‘real life’ phage therapy suitability

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dc.contributor.authorCasey, Eoghan
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorMahony, Jennifer
dc.contributor.funderScience Foundation Ireland
dc.contributor.funderBill and Melinda Gates Foundation
dc.contributor.funderSeventh Framework Programme
dc.date.accessioned2018-05-02T10:16:31Z
dc.date.available2018-05-02T10:16:31Z
dc.date.issued2018
dc.description.abstractThe increasing problem of antibiotic-resistant pathogens has put enormous pressure on healthcare providers to reduce the application of antibiotics and to identify alternative therapies. Phages represent such an alternative with significant application potential, either on their own or in combination with antibiotics to enhance the effectiveness of traditional therapies. However, while phage therapy may offer exciting therapeutic opportunities, its evaluation for safe and appropriate use in humans needs to be guided initially by reliable and appropriate assessment techniques at the laboratory level. Here, we review the process of phage isolation and the application of individual pathogens or reference collections for the development of specific or “off-the-shelf” preparations. Furthermore, we evaluate current characterization approaches to assess the in vitro therapeutic potential of a phage including its spectrum of activity, genome characteristics, storage and administration requirements and effectiveness against biofilms. Lytic characteristics and the ability to overcome anti-phage systems are also covered. These attributes direct phage selection for their ultimate application as antimicrobial agents. We also discuss current pitfalls in this research area and propose that priority should be given to unify current phage characterization approaches.en
dc.description.sponsorshipEU Joint Programming Initiative—A Healthy Diet for a Healthy Life (JPI HDHL, http://www.healthydietforhealthylife.eu/); Bill and Melinda Gates Foundation (Grand Challenges xplorations scheme (Ref. No. OPP1150567)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid163
dc.identifier.citationCasey, E., van Sinderen, D. and Mahony, J. (2018) 'In vitro characteristics of phages to guide ‘real life’ phage therapy suitability', Viruses, 10(4), 163 (20pp). doi: 10.3390/v10040163en
dc.identifier.doi10.3390/v10040163
dc.identifier.endpage20
dc.identifier.issn1999-4915
dc.identifier.issued4
dc.identifier.journaltitleVirusesen
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/5970
dc.identifier.volume10
dc.language.isoenen
dc.publisherMDPI AGen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2273/IE/Alimentary Pharmabiotic Centre (APC) - Interfacing Food & Medicine/
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Starting Investigator Research Grant (SIRG)/15/SIRG/3430/IE/Phage-host interactome of the dairy bacterium Streptococcus thermophilus (PHIST)/
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/13/IA/1953/IE/Functional analysis of the host adsorption and DNA injection processes of a lactococcal bacteriophage/
dc.relation.projectinfo:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/277673/EU/A Healthy Diet for a Healthy Life - Coordination Action/HDHL CSA
dc.relation.urihttp://www.mdpi.com/1999-4915/10/4/163
dc.rights© 2018, the Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectpH stabilityen
dc.subjectPhage-host interactionsen
dc.subjectGenomicsen
dc.subjectAntibiotic-resistanceen
dc.subjectPhage preparationen
dc.subjectLysinsen
dc.subjectBiofilmsen
dc.titleIn vitro characteristics of phages to guide ‘real life’ phage therapy suitabilityen
dc.typeArticle (peer-reviewed)en
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