Effects of endurance racing on horse plasma extracellular particle miRNA

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dc.contributor.authorde Oliveira Jr, GetĂșlio P.
dc.contributor.authorPorto, William F.
dc.contributor.authorPalu, Cintia C.
dc.contributor.authorPereira, Lydyane M.
dc.contributor.authorReis, Alessandra M. M.
dc.contributor.authorMarçola, Tatiana G.
dc.contributor.authorTeixeira-Neto, Antonio R.
dc.contributor.authorFranco, Octavio L.
dc.contributor.authorPereira, Rinaldo W.
dc.contributor.funderCoordenação de Aperfeiçoamento de Pessoal de Nível Superioren
dc.contributor.funderConselho Nacional de Desenvolvimento CientĂ­fico e TecnolĂłgicoen
dc.contributor.funderFundação de Apoio à Pesquisa do Distrito Federalen
dc.date.accessioned2021-11-09T14:42:11Z
dc.date.available2021-11-09T14:42:11Z
dc.date.issued2020-06-02
dc.description.abstractBackground: Physical exercise is an essential factor in preventing and treating metabolic diseases by promoting systemic benefits throughout the body. The molecular factors involved in this process are poorly understood. Micro RNAs (miRNAs) are small non-coding RNAs that inhibit mRNA transcription. MiRNAs, which can participate in the benefits of exercise to health, circulate in plasma in extracellular particles (EP). Horses that undergo endurance racing are an excellent model to study the impact of long-duration/low intensity exercise in plasma EP miRNAs. Objectives: To evaluate the effects of 160 km endurance racing on horse plasma extracellular particles and their miRNA population. Study design: Cohort study. Methods: We collected plasma from five Arabian horses during five time-points of an endurance ride. Extracellular particles were purified from plasma and characterised by electron microscopy, resistive pulse sensing (qNano) and western blotting. Small RNAs were purified from horse plasma EP, and sequencing was performed. Results: Endurance racing increased EP concentration and average diameter compared to before the race. Western blotting showed a high concentration of extracellular vesicles proteins 2 hours after the race, which returned to baseline 15 hours after the race. MicroRNA differential expression analysis revealed increasing levels of eca-miR-486-5p during and after the race, and decreasing levels of eca-miR-9083 after the end. Conclusions: This study adds new data about the variation in plasma EP concentrations after long-distance exercise and brings new insights about the roles of exercise-derived EP miRNAs during low-intensity endurance exercise.en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationde Oliveira, G. P., Porto, W. F., Palu, C.C., Pereira, L. M., Reis, A. M. M., Marçola, T. G., Teixeira-Neto, A. R., Franco, O. L. and Pereira, R. W. (2021) 'Effects of endurance racing on horse plasma extracellular particle miRNA', Equine Veterinary Journal, 53(3), pp. 618-627. doi: 10.1111/evj.13300en
dc.identifier.doi10.1111/evj.13300en
dc.identifier.eissn2042-3306
dc.identifier.endpage627en
dc.identifier.issn0425-1644
dc.identifier.issued3en
dc.identifier.journaltitleEquine Veterinary Journalen
dc.identifier.startpage618en
dc.identifier.urihttps://hdl.handle.net/10468/12175
dc.identifier.volume53en
dc.language.isoenen
dc.publisherJohn Wiley & Sons, Inc.en
dc.rights© 2020, Equine Veterinary Journal. Published by John Wiley & Sons, Inc. This is the peer reviewed version of the following item: de Oliveira, G. P., Porto, W. F., Palu, C.C., Pereira, L. M., Reis, A. M. M., Marçola, T. G., Teixeira-Neto, A. R., Franco, O. L. and Pereira, R. W. (2021) 'Effects of endurance racing on horse plasma extracellular particle miRNA', Equine Veterinary Journal, 53(3), pp. 618-627, doi: 10.1111/evj.13300, which has been published in final form at: https://doi.org/10.1111/evj.13300 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en
dc.subjectHorseen
dc.subjectEndurance racingen
dc.subjectExtracellular particlesen
dc.subjectEPen
dc.subjectMicroRNAen
dc.subjectmiRNAen
dc.titleEffects of endurance racing on horse plasma extracellular particle miRNAen
dc.typeArticle (peer-reviewed)en
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