Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches

dc.contributor.authorLugli, Gabriele Andrea
dc.contributor.authorMilani, Christian
dc.contributor.authorDuranti, Sabrina
dc.contributor.authorAlessandri, Giulia
dc.contributor.authorTurroni, Francesca
dc.contributor.authorMancabelli, Leonardo
dc.contributor.authorTatoni, Danilo
dc.contributor.authorOssiprandi, Maria Cristina
dc.contributor.authorvan Sinderen, Douwe
dc.contributor.authorVentura, Marco
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderEuropean Commissionen
dc.date.accessioned2019-11-20T05:31:13Z
dc.date.available2019-11-20T05:31:13Z
dc.date.issued2019-05-16
dc.description.abstractWhole metagenome shotgun (WMGS) sequencing is a method that provides insights into the genomic composition and arrangement of complex microbial consortia. Here, we report how WMGS coupled with a cultivation approach allows the isolation of novel bifidobacteria from animal fecal samples. A combination of in silico analyses based on nucleotide and protein sequences facilitate the identification of genetic material belonging to putative novel species. Consequently, the prediction of metabolic properties by in silico analyses permits the identification of specific substrates that are then employed to isolate these species through a cultivation method.en
dc.description.sponsorshipScience Foundation Ireland (15/JP-HDHL/3280)en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid96en
dc.identifier.citationLugli, G.A., Milani, C., Duranti, S., Alessandri, G., Turroni, F., Mancabelli, L., Tatoni, D., Ossiprandi, M.C., van Sinderen, D. and Ventura, M. (2019). 'Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches'. Genome biology, 20(1), 96. (6pp.) DOI:10.1186/s13059-019-1711-6en
dc.identifier.doi10.1186/s13059-019-1711-6en
dc.identifier.eissn1465-6906
dc.identifier.endpage6en
dc.identifier.issn1474-760X
dc.identifier.issued1en
dc.identifier.journaltitleGenome Biologyen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/9129
dc.identifier.volume20en
dc.language.isoenen
dc.publisherBioMed Central Ltd.en
dc.relation.projectinfo:eu-repo/grantAgreement/EC/H2020::CSA/696300/EU/The second coordination and support action for the JPI Healthy Diet for a Healthy Life/CSA JPI HDHL 2.0en
dc.relation.urihttps://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1711-6
dc.rights© The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectGenomicsen
dc.subjectMetagenomicsen
dc.subjectMicrobiotaen
dc.subjectHuman gut commensalsen
dc.titleIsolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approachesen
dc.typeArticle (peer-reviewed)en
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