Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches
dc.contributor.author | Lugli, Gabriele Andrea | |
dc.contributor.author | Milani, Christian | |
dc.contributor.author | Duranti, Sabrina | |
dc.contributor.author | Alessandri, Giulia | |
dc.contributor.author | Turroni, Francesca | |
dc.contributor.author | Mancabelli, Leonardo | |
dc.contributor.author | Tatoni, Danilo | |
dc.contributor.author | Ossiprandi, Maria Cristina | |
dc.contributor.author | van Sinderen, Douwe | |
dc.contributor.author | Ventura, Marco | |
dc.contributor.funder | Science Foundation Ireland | en |
dc.contributor.funder | European Commission | en |
dc.date.accessioned | 2019-11-20T05:31:13Z | |
dc.date.available | 2019-11-20T05:31:13Z | |
dc.date.issued | 2019-05-16 | |
dc.description.abstract | Whole metagenome shotgun (WMGS) sequencing is a method that provides insights into the genomic composition and arrangement of complex microbial consortia. Here, we report how WMGS coupled with a cultivation approach allows the isolation of novel bifidobacteria from animal fecal samples. A combination of in silico analyses based on nucleotide and protein sequences facilitate the identification of genetic material belonging to putative novel species. Consequently, the prediction of metabolic properties by in silico analyses permits the identification of specific substrates that are then employed to isolate these species through a cultivation method. | en |
dc.description.sponsorship | Science Foundation Ireland (15/JP-HDHL/3280) | en |
dc.description.status | Peer reviewed | en |
dc.description.version | Published Version | en |
dc.format.mimetype | application/pdf | en |
dc.identifier.articleid | 96 | en |
dc.identifier.citation | Lugli, G.A., Milani, C., Duranti, S., Alessandri, G., Turroni, F., Mancabelli, L., Tatoni, D., Ossiprandi, M.C., van Sinderen, D. and Ventura, M. (2019). 'Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches'. Genome biology, 20(1), 96. (6pp.) DOI:10.1186/s13059-019-1711-6 | en |
dc.identifier.doi | 10.1186/s13059-019-1711-6 | en |
dc.identifier.eissn | 1465-6906 | |
dc.identifier.endpage | 6 | en |
dc.identifier.issn | 1474-760X | |
dc.identifier.issued | 1 | en |
dc.identifier.journaltitle | Genome Biology | en |
dc.identifier.startpage | 1 | en |
dc.identifier.uri | https://hdl.handle.net/10468/9129 | |
dc.identifier.volume | 20 | en |
dc.language.iso | en | en |
dc.publisher | BioMed Central Ltd. | en |
dc.relation.project | info:eu-repo/grantAgreement/EC/H2020::CSA/696300/EU/The second coordination and support action for the JPI Healthy Diet for a Healthy Life/CSA JPI HDHL 2.0 | en |
dc.relation.uri | https://genomebiology.biomedcentral.com/articles/10.1186/s13059-019-1711-6 | |
dc.rights | © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | Genomics | en |
dc.subject | Metagenomics | en |
dc.subject | Microbiota | en |
dc.subject | Human gut commensals | en |
dc.title | Isolation of novel gut bifidobacteria using a combination of metagenomic and cultivation approaches | en |
dc.type | Article (peer-reviewed) | en |
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