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E-thesis on CORA only Entire Thesis Restricted No This thesis is due for publication or the author is actively seeking to publish this material Hill, Colin Begley, Máire Morrissey, Ruth M. Department of Agriculture, Food and the Marine, Ireland 2014-02-19T14:37:13Z 2015-02-20T05:00:05Z 2013 2013 Bioluminescence is the production of light by living organisms as a result of a number of enzyme catalysed reactions caused by enzymes termed luciferases. The lux genes responsible for the emission of light can be cloned from one bioluminescent microorganism into one that is not bioluminescent. The light emitted can be monitored and quantified and will provide information on the metabolic activity, quantity and location of cells in a particular environment, in real-time. The primary aim of this thesis was to investigate and identify several food industry related applications of lux-tagged microorganisms. The first aim was to monitor a lux-tagged Cronobacter sakazakii in reconstituted infant milk formula, in realtime. The second aim was to investigate a bioluminescent-based early warning system for starter culture disruption by bacteriophages and antibiotic residues. The third of this thesis was to examine the use of a bioluminescent-based assay to test the activity of bioengineered Nisin derivatives M21V and S29A against foodborne pathogens in laboratory media and selected foods. Department of Agriculture, Food and the Marine, Ireland (Food Institutional Research Measure (08RDC602)) Not peer reviewed Accepted Version application/pdf Morrissey, R. M. 2013. Shining light on food microbiology; applications of luciferase-tagged microorganisms in the food industry. PhD Thesis, University College Cork. 182 https://hdl.handle.net/10468/1395 en University College Cork © 2013, Ruth M. Morrissey http://creativecommons.org/licenses/by-nc-nd/3.0/ Luciferase Luciferases Bioluminescence Food--Microbiology false Shining light on food microbiology; applications of luciferase-tagged microorganisms in the food industry Doctoral thesis Doctoral PhD (Science) c.hill@ucc.ieinfo:eu-repo/semantics/openAccess