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Not applicable No embargo required Not applicable No embargo required No Embargo Required O'Gara, Fergal Egan, Michael Frank Higher Education Authority 2016-06-22T09:22:40Z 2016-06-22T09:22:40Z 2014 2014 Direct secretion systems which deliver molecules from one cell to another have huge significance in shaping bacterial communities or in determining the outcome of bacterial associations with eukaryotic organisms. This work examines the roles of the Type III Secretion System (T3SS) and the Type VI Secretion System (T6SS) systems of Pseudomonas, a widespread genus including clinical pathogens and biocontrol strains. Bioinformatic analysis of T6SS phylogeny and associated gene content within Pseudomonas identified several T6SS phylogenetic groups, and linked T6SS components VgrG and Hcp encoded outside of T6SS gene loci with their cognate T6SS phylogenetic groups. Remarkably, such “orphan” vgrG and hcp genes were found to occur in diverse, horizontally transferred, operons often containing putative T6SS accessory components and effectors. The prevalence of a widespread superfamily of T6SS lipase effectors (Tle) was assessed in metagenomes from various environments. The abundance of the Tle superfamily and individual families varied between niches, suggesting there is niche specific selection and specialisation of Tle. Experimental work also discovered that P. fluorescens F113 uses the SPI-1 T3SS to avoid amoeboid grazing in mixed populations. This finding may represent a significant aspect of F113 rhizocompetence, and the rhizocompetence of other Rhizobacteria. Higher Education Authority (PRTLI4) Not peer reviewed Accepted Version application/pdf Egan, M.F. 2014. An analysis of selected secretion systems of pseudomonas species. PhD Thesis, University College Cork 258 https://hdl.handle.net/10468/2771 en University College Cork © 2014, Michael Frank Egan. http://creativecommons.org/licenses/by-nc-nd/3.0/ Pseudomonas Type VI secretion system Type III secretion system Type VI lipase effectors VgrG islands false An analysis of selected secretion systems of Pseudomonas species Doctoral thesis Doctoral PhD (Science) f.ogara@ucc.ieinfo:eu-repo/semantics/openAccess