In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates

Simple item page
dc.contributor.authorCermeño, Maria
dc.contributor.authorFitzGerald, Richard J.
dc.contributor.authorO'Brien, Nora M.
dc.contributor.funderDepartment of Agriculture, Food and the Marine, Ireland
dc.date.accessioned2016-09-21T08:40:10Z
dc.date.available2016-09-21T08:40:10Z
dc.date.issued2016-08-31
dc.description.abstractSodium caseinate (NaCN) was incubated prior to and after hydrolysis with a microbial transglutaminase (TGase) and hydrolysed with Prolyve 1000. The resultant hydrolysates were tested for their immunomodulatory and antioxidant activity. TGase-treated hydrolysates significantly reduced (p < 0.05) the production of IL-6 at 0.5 and 1 mg mL−1 and the non-TGase treated hydrolysate reduced the production of IL-6 at 1 mg mL−1 in concanavalin (ConA) stimulated Jurkat T cells. None of the samples had an effect on IL-2. The hydrolysates showed higher oxygen radical absorbance capacity assay and ferric reducing antioxidant power activity than unhydrolysed NaCN, but no significant (p > 0.05) differences were found between the TGase-treated and non-TGase-treated samples. In the presence of hydrogen peroxide, the non-TGase-treated sample exhibited the highest DNA protective effect in U937 cells. These findings suggest that NaCN derived hydrolysates with and without treatment with TGase may exert specific antioxidant, genoprotective and anti-inflammatory effects.en
dc.description.sponsorshipDepartment of Agriculture, Food and the Marine, Ireland (Food Institutional Research Measure (FIRM) Grant Number: 10 RD TMFRC 701)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationCermeño, M., FitzGerald, R.J. and O’Brien, N.M. (2016) 'In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates', International Dairy Journal, 63, pp. 107-114. doi: 10.1016/j.idairyj.2016.08.007en
dc.identifier.endpage114en
dc.identifier.issn0958-6946
dc.identifier.journaltitleInternational Dairy Journalen
dc.identifier.startpage107en
dc.identifier.urihttps://hdl.handle.net/10468/3104
dc.identifier.volume63en
dc.language.isoenen
dc.publisherElsevier B.V.en
dc.rights© 2016, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
dc.subjectTGaseen
dc.subjectGenoprotectiveen
dc.subjectDNAen
dc.subjectIL-6 cytokineen
dc.subjectAnti-inflammatoryen
dc.subjectFractionationen
dc.subjectIsolationen
dc.subjectPeptidesen
dc.subjectBioactive hydrolysatesen
dc.titleIn vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysatesen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1195.pdf
Size:
706.96 KB
Format:
Adobe Portable Document Format
Description:
Accepted Version
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Food and Nutritional Sciences - Journal Articles