Access to this article is restricted until 12 months after publication by request of the publisher.. Restriction lift date: 2017-08-31
In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates
| dc.check.date | 2017-08-31 | |
| dc.check.info | Access to this article is restricted until 12 months after publication by request of the publisher. | en |
| dc.contributor.author | Cermeño, Maria | |
| dc.contributor.author | FitzGerald, Richard J. | |
| dc.contributor.author | O'Brien, Nora M. | |
| dc.contributor.funder | Department of Agriculture, Food and the Marine, Ireland | |
| dc.date.accessioned | 2016-09-21T08:40:10Z | |
| dc.date.available | 2016-09-21T08:40:10Z | |
| dc.date.issued | 2016-08-31 | |
| dc.description.abstract | Sodium caseinate (NaCN) was incubated prior to and after hydrolysis with a microbial transglutaminase (TGase) and hydrolysed with Prolyve 1000. The resultant hydrolysates were tested for their immunomodulatory and antioxidant activity. TGase-treated hydrolysates significantly reduced (p < 0.05) the production of IL-6 at 0.5 and 1 mg mL−1 and the non-TGase treated hydrolysate reduced the production of IL-6 at 1 mg mL−1 in concanavalin (ConA) stimulated Jurkat T cells. None of the samples had an effect on IL-2. The hydrolysates showed higher oxygen radical absorbance capacity assay and ferric reducing antioxidant power activity than unhydrolysed NaCN, but no significant (p > 0.05) differences were found between the TGase-treated and non-TGase-treated samples. In the presence of hydrogen peroxide, the non-TGase-treated sample exhibited the highest DNA protective effect in U937 cells. These findings suggest that NaCN derived hydrolysates with and without treatment with TGase may exert specific antioxidant, genoprotective and anti-inflammatory effects. | en |
| dc.description.sponsorship | Department of Agriculture, Food and the Marine, Ireland (Food Institutional Research Measure (FIRM) Grant Number: 10 RD TMFRC 701) | en |
| dc.description.status | Peer reviewed | en |
| dc.description.version | Accepted Version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Cermeño, M., FitzGerald, R.J. and O’Brien, N.M. (2016) 'In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates', International Dairy Journal, 63, pp. 107-114. doi: 10.1016/j.idairyj.2016.08.007 | en |
| dc.identifier.endpage | 114 | en |
| dc.identifier.issn | 0958-6946 | |
| dc.identifier.journaltitle | International Dairy Journal | en |
| dc.identifier.startpage | 107 | en |
| dc.identifier.uri | https://hdl.handle.net/10468/3104 | |
| dc.identifier.volume | 63 | en |
| dc.language.iso | en | en |
| dc.publisher | Elsevier B.V. | en |
| dc.rights | © 2016, Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 license. | en |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
| dc.subject | TGase | en |
| dc.subject | Genoprotective | en |
| dc.subject | DNA | en |
| dc.subject | IL-6 cytokine | en |
| dc.subject | Anti-inflammatory | en |
| dc.subject | Fractionation | en |
| dc.subject | Isolation | en |
| dc.subject | Peptides | en |
| dc.subject | Bioactive hydrolysates | en |
| dc.title | In vitro antioxidant and immunomodulatory activity of transglutaminase-treated sodium caseinate hydrolysates | en |
| dc.type | Article (peer-reviewed) | en |