Exploiting the bioactive potential of marine sponge microbiota

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dc.contributor.advisor Morrissey, John P. en
dc.contributor.advisor O'Gara, Fergal en
dc.contributor.advisor Barbosa, Teresa M. en
dc.contributor.author Phelan, Robert W.
dc.date.accessioned 2014-03-25T10:18:32Z
dc.date.issued 2013
dc.date.submitted 2013
dc.identifier.citation Phelan, R. W. 2013. Exploiting the bioactive potential of marine sponge microbiota. PhD Thesis, University College Cork. en
dc.identifier.uri http://hdl.handle.net/10468/1489
dc.description.abstract Endospore-forming bacteria are often isolated from different marine sponges, but their abundance varies, and they are frequently missed by culture-independent studies. Within endospore-formers, Bacillus are renowned for the production of antimicrobials and other compounds of medical and industrial importance. Although this group has been well studied in many different environments, very little is known about the actual diversity and properties of sporeformers associated with marine sponges. Identification of the endospore-forming bacteria associated with the marine sponges; Haliclona simulans, Amphilectus fucorum and Cliona celata, has uncovered an abundant and diverse microbial population composed of Bacillus, Paenibacillus, Solibacillus, Halobacillus and Viridibacillus species. This diversity appears to be overlooked by other non-targeted approaches where spore-formers are masked by more dominant species within the ecosystem. In addition to the identification of two antibiotic resistant plasmids, this bank of sporeformers produce a range of bioactive compounds. New antimicrobial compounds are urgently needed to combat the spread of multidrug resistant pathogens, as few new options are entering the drug discovery pipelines for clinical trials. Based on the results of this project, endospore-formers associated with marine sponges may hold the answer. The power of coupling functional based assays with genomic approaches has enabled us to identify a novel class 1 lantibiotic, subtilomycin, which is active against several clinically relevant pathogens. Subtilomycin is encoded in the genomes of all the marine sponge B. subtilis isolates analysed. They cluster together phylogenetically and form a distinct group from other sequenced B. subtilis strains. Regardless of its potential clinical relevance, subtilomycin may be providing these strains with a specific competitive advantage(s) within the stringent confines of the marine sponge environment. This work has outlined the industrial and biotechnological potential of marine sponge endospore-formers which appear to produce a cocktail of bioactive compounds. Genome sequencing of specific marine sponge isolates highlighted the importance of mining extreme environments and habitats for new lead compounds with potential therapeutic applications. en
dc.description.sponsorship (Beaufort Marine Biodiscovery Award C & CRA 2007/082) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2013, Robert W. Phelan en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Marine sponges en
dc.subject Bacillus subtilis en
dc.subject Lantibiotics en
dc.subject Antibiotic resistance en
dc.subject Endospore-formers en
dc.subject Antimicrobial activity en
dc.subject Subtilomycin en
dc.subject.lcsh Sponges en
dc.subject.lcsh Bacillus subtilis en
dc.title Exploiting the bioactive potential of marine sponge microbiota en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text not available en
dc.check.info Indefinite en
dc.check.date 10000-01-01
dc.description.version Accepted Version
dc.contributor.funder Marine Institute en
dc.description.status Not peer reviewed en
dc.internal.school Microbiology en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Yes en
dc.thesis.opt-out true
dc.check.embargoformat Not applicable en

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