MicroRNA expression and function in neonatal hypoxic ischaemic encephalopathy

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dc.contributor.advisor Murray, Deirdre M. en
dc.contributor.advisor Boylan, Geraldine B. en
dc.contributor.advisor Cryan, John F. en
dc.contributor.author Looney, Ann-Marie
dc.date.accessioned 2016-09-21T11:23:28Z
dc.date.available 2016-09-21T11:23:28Z
dc.date.issued 2016
dc.date.submitted 2016
dc.identifier.citation Looney, A. 2016. MicroRNA expression and function in neonatal hypoxic ischaemic encephalopathy. PhD Thesis, University College Cork. en
dc.identifier.uri http://hdl.handle.net/10468/3107
dc.description.abstract Hypoxic ischaemic encephalopathy (HIE) is a devastating neonatal condition which affects 2-3 per 1000 infants annually. The current gold standard of treatment - induced hypothermia, has the ability to reduce neonatal mortality and improve neonatal morbidity. However, to be effective it needs to be initiated within the therapeutic window which exists following initial insult until approximately 6 hours after birth. Current methods of assessment which are relied upon to identify infants with HIE are subjective and unreliable. To overcome this issue, an early and reliable biomarker of HIE severity must be identified. MicroRNA (miRNA) are a class of small non-coding RNA molecules which have potential as biomarkers of disease state and potential therapeutic targets. These tiny molecules can modulate gene expression by inhibiting translation of messenger RNA (mRNA) and as a result, can regulate protein synthesis. These miRNA are understood to be released into the circulation during cellular stress, where they are highly stable and relatively easy to quantify. Therefore, these miRNAs may be ideal candidates for biomarkers of HIE severity and may aid in directing the clinical management of these infants. By using both transcriptomic and proteomic approaches to analyse the expression of miRNAs and their potential targets in the umbilical cord blood, I have confirmed that infants with perinatal asphyxia and HIE have a significantly different UCB miRNA signature compared to UCB samples from healthy controls. Finally, I have identified and investigated 2 individual miRNAs; both of which show some potential as classifiers of HIE severity and predictors of long term outcome, particularly when coupled with their downstream targets. While this work will need to be validated and expanded in a new and larger cohort of infants, it suggests the potential of miRNA as biomarkers of neonatal pathological conditions such as HIE. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2016, Ann-Marie Looney. en
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/ en
dc.subject Biomarker en
dc.subject MicroRNA en
dc.subject Hypoxic ischaemic encephalopathy en
dc.subject Neurodevelopmental outcome en
dc.title MicroRNA expression and function in neonatal hypoxic ischaemic encephalopathy en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral Degree (Structured) en
dc.type.qualificationname PhD (Medicine and Health) en
dc.internal.availability Full text not available en
dc.check.info No embargo required en
dc.description.version Accepted Version
dc.contributor.funder Health Research Board en
dc.description.status Not peer reviewed en
dc.internal.school Paediatrics and Child Health en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Yes en
dc.thesis.opt-out true
dc.check.embargoformat Not applicable en
dc.internal.conferring Autumn 2016 en

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© 2016, Ann-Marie Looney. Except where otherwise noted, this item's license is described as © 2016, Ann-Marie Looney.
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