Cardiopulmonary bypass down-regulates NOD signaling and inflammatory response in children with congenital heart disease

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dc.contributor.author Yang, Qinghua
dc.contributor.author Liao, Jianyi
dc.contributor.author Huang, Jie
dc.contributor.author Li, Yi Ping
dc.contributor.author Huang, Shungen
dc.contributor.author Zhou, Huiting
dc.contributor.author Xie, Yi
dc.contributor.author Pan, Jian
dc.contributor.author Li, Yanhong
dc.contributor.author Wang, Jiang Huai
dc.contributor.author Wang, Jian
dc.date.accessioned 2016-10-10T11:12:20Z
dc.date.available 2016-10-10T11:12:20Z
dc.date.issued 2016-09-13
dc.identifier.citation Yang, Q., Liao, J., Huang, J., Li, Y.P., Huang, S., Zhou, H., Xie, Y., Pan, J., Li, Y., Wang, J.H. and Wang, J. (2016) 'Cardiopulmonary bypass down-regulates NOD signaling and inflammatory response in children with congenital heart disease', PLoS ONE, 11(9), e0162179 (11pp). doi:10.1371/journal.pone.0162179 en
dc.identifier.volume 11 en
dc.identifier.issued 9 en
dc.identifier.startpage 1 en
dc.identifier.endpage 11 en
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10468/3171
dc.identifier.doi 10.1371/journal.pone.0162179
dc.description.abstract In the present study, we aimed to examine the impact of cardiopulmonary bypass (CPB) on expression and function of NOD1 and NOD2 in children with congenital heart disease (CHD), in an attempt to clarify whether NOD1 and NOD2 signaling is involved in the modulation of host innate immunity against postoperative infection in pediatric CHD patients. Peripheral blood samples were collected from pediatric CHD patients at five different time points: before CPB, immediately after CPB, and 1, 3, and 7 days after CPB. Real-time PCR, Western blot, and ELISA were performed to measure the expression of NOD1 and NOD2, their downstream signaling pathways, and inflammatory cytokines at various time points. Proinflammatorycytokine IL-6 and TNF-α levels in response to stimulation with either the NOD1 agonist Tri-DAP or the NOD2 agonist MDP were significantly reduced after CPB compared with those before CPB, which is consistent with a suppressed inflammatory response postoperatively. The expression of phosphorylated RIP2 and activation of the downstream signaling pathways NF-κB p65 and MAPK p38 upon Tri-DAP or MDP stimulation in PBMCs were substantially inhibited after CPB. The mRNA level of NOD1 and protein levels of NOD1 and NOD2 were also markedly decreased after CPB. Our results demonstrated that NOD-mediated signaling pathways were substantially inhibited after CPB, which correlates with the suppressed inflammatory response and may account, at least in part, for the increased risk of postoperative infection in pediatric CHD patients. en
dc.description.sponsorship National Natural Science Foundation of China (Grants 81272143 and 81420108022); National Natural Science Foundation of Jiangsu Province, China (Grant BK2011310); Innovative Team of Jiangsu Province, China (Grant LJ201141), and Jiangsu Province Program of Innovative and Entrepreneurial Talents, China (2011-2014) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Public Library of Science en
dc.rights © 2016 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Congenital heart disease en
dc.subject NOD1 en
dc.subject NOD2 en
dc.subject Immunity en
dc.subject Postoperative infection en
dc.title Cardiopulmonary bypass down-regulates NOD signaling and inflammatory response in children with congenital heart disease en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Jiang Huai Wang, Surgery, University College Cork, Cork, Ireland. +353-21-490-3000 Email: jh.wang@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder National Natural Science Foundation of China
dc.contributor.funder National Natural Science Foundation of Jiangsu Province, China
dc.contributor.funder Innovative Team of Jiangsu Province, China
dc.contributor.funder Jiangsu Province Program of Innovative and Entrepreneurial Talents, China
dc.description.status Peer reviewed en
dc.identifier.journaltitle PLoS ONE en
dc.internal.IRISemailaddress jh.wang@ucc.ie
dc.internal.IRISemailaddress jh.wang@ucc.ie en
dc.identifier.articleid e0162179


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© 2016 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Except where otherwise noted, this item's license is described as © 2016 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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