Investigation of the presence and activity of the innate immune component, Complement, in bovine milk

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dc.contributor.advisor Stanton, Catherine en
dc.contributor.advisor Kelly, Philip M. en
dc.contributor.advisor Fitzgerald, Gerald F. en Maye, Susan 2017-01-24T13:39:18Z 2017-01-24T13:39:18Z 2016 2016
dc.identifier.citation Maye, S. 2016. Investigation of the presence and activity of the innate immune component, Complement, in bovine milk. PhD Thesis, University College Cork. en
dc.identifier.endpage 211 en
dc.description.abstract The occurrence of Complement in human milk indicates the presence of innate immune components of maternal origin and their putative defensive role in the neonatal gut. In order to ascertain the status of Complement in bovine milk, it was necessary to validate the suitability of the Complement-sensitive bacterial sequestration assay used to monitor human milk. The relative bacteriostatic effects, expressed as a differential between the initial and final assay counts following inhibition of the E. coli O111 marker strain were, 6.20 and 6.06 log CFU/ml for raw bovine and human milks, respectively. Lower levels of Complement activity measured in pasteurised and low-fat milks were observed during the course of heat-inactivation studies, while gravity separation of cream over a 24 h period confirmed a greater degree of attachment by Complement to the rising cream layer. The E. coli O111 sequestration assay strain had restricted growth of 7.5 and 8.2 CFU/ml in the higher and lower gravity-separated fractions, respectively. An animal health effect was observed, with Complement appearing to be more active in milk samples from cows with a higher somatic cell counts (SCC). Inducing sub-clinical mastitis in the healthy quarter of a lactating cow confirmed that increased Complement activity effect was local to the affected quarter and not manifested systemically. Complement activity levels varied in the milks of 7 commercially important prominent dairy breeds and crossbreeds surveyed. Both the Norwegian Red and Kerry breed cows stood out as having higher Complement activity (6.25 and 6.92 CFU/ml, respectively); these two breeds are known to have stronger immunity and a better capacity to resist mastitic infection. The findings of this study have implications for both milk production and processing disciplines. In order to retain the antimicrobial efficacy associated with xi Complement in raw bovine milk, milder process treatments than hitherto practised in industry should be adopted. en
dc.description.sponsorship Teagasc (Walsh Fellowship Scheme) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2016, Susan Maye. en
dc.rights.uri en
dc.subject Complement en
dc.subject Bovine milk en
dc.subject Antimicrobial en
dc.subject Bovine en
dc.subject E.coli O111 en
dc.title Investigation of the presence and activity of the innate immune component, Complement, in bovine milk en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD (Science) en
dc.internal.availability Full text available en No embargo required en
dc.description.version Accepted Version
dc.contributor.funder Teagasc en
dc.description.status Not peer reviewed en Microbiology en Teagasc en
dc.check.type No Embargo Required
dc.check.reason No embargo required en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Not applicable en
dc.internal.conferring Spring 2017 en

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© 2016, Susan Maye. Except where otherwise noted, this item's license is described as © 2016, Susan Maye.
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