A nascent peptide signal responsive to endogenous levels of polyamines acts to stimulate regulatory frameshifting on antizyme mRNA.

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dc.contributor.author Yordanova, Martina M.
dc.contributor.author Wu, Cheng
dc.contributor.author Andreev, Dmitry E.
dc.contributor.author Sachs, Matthew S.
dc.contributor.author Atkins, John F.
dc.date.accessioned 2018-03-29T09:34:46Z
dc.date.available 2018-03-29T09:34:46Z
dc.date.issued 2015-05-21
dc.identifier.citation Yordanova, M. M., Wu, C., Andreev, D. E., Sachs, M. S. and Atkins, J. F. (2015) 'A Nascent Peptide Signal Responsive to Endogenous Levels of Polyamines Acts to Stimulate Regulatory Frameshifting on Antizyme mRNA', Journal of Biological Chemistry, 290(29), pp. 17863-17878. doi: 10.1074/jbc.M115.647065 en
dc.identifier.volume 290 en
dc.identifier.issued 29 en
dc.identifier.startpage 17863 en
dc.identifier.endpage 17878 en
dc.identifier.issn 0021-9258
dc.identifier.uri http://hdl.handle.net/10468/5717
dc.identifier.doi 10.1074/jbc.M115.647065
dc.description.abstract The protein antizyme is a negative regulator of cellular polyamine concentrations from yeast to mammals. Synthesis of functional antizyme requires programmed +1 ribosomal frameshifting at the 3' end of the first of two partially overlapping ORFs. The frameshift is the sensor and effector in an autoregulatory circuit. Except for Saccharomyces cerevisiae antizyme mRNA, the frameshift site alone only supports low levels of frameshifting. The high levels usually observed depend on the presence of cis-acting stimulatory elements located 5' and 3' of the frameshift site. Antizyme genes from different evolutionary branches have evolved different stimulatory elements. Prior and new multiple alignments of fungal antizyme mRNA sequences from the Agaricomycetes class of Basidiomycota show a distinct pattern of conservation 5' of the frameshift site consistent with a function at the amino acid level. As shown here when tested in Schizosaccharomyces pombe and mammalian HEK293T cells, the 5' part of this conserved sequence acts at the nascent peptide level to stimulate the frameshifting, without involving stalling detectable by toe-printing. However, the peptide is only part of the signal. The 3' part of the stimulator functions largely independently and acts at least mostly at the nucleotide level. When polyamine levels were varied, the stimulatory effect was seen to be especially responsive in the endogenous polyamine concentration range, and this effect may be more general. A conserved RNA secondary structure 3' of the frameshift site has weaker stimulatory and polyamine sensitizing effects on frameshifting. en
dc.description.sponsorship National Institutes of Health (Grant GM068087) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher American Society for Biochemistry and Molecular Biology en
dc.relation.uri http://www.jbc.org/content/290/29/17863.abstract
dc.rights © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version free via Creative Commons CC-BY license. en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject mRNA en
dc.subject Polyamine en
dc.subject Ribosome en
dc.subject RNA structure en
dc.subject Translation regulation en
dc.subject ORF en
dc.subject Antizyme en
dc.subject Frameshifting en
dc.title A nascent peptide signal responsive to endogenous levels of polyamines acts to stimulate regulatory frameshifting on antizyme mRNA. en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John Atkins, Biochemistry, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.atkins@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2018-03-29T09:28:58Z
dc.description.version Published Version en
dc.internal.rssid 312371114
dc.internal.pmid 25998126
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder National Institutes of Health en
dc.description.status Peer reviewed en
dc.identifier.journaltitle The Journal of Biological Chemistry en
dc.internal.copyrightchecked No !!CORA!! en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress j.atkins@ucc.ie en


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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version free via Creative Commons CC-BY license. Except where otherwise noted, this item's license is described as © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version free via Creative Commons CC-BY license.
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