Intrarenal Mas and AT(1) receptors play a role in mediating the excretory actions of renal interstitial angiotensin-(1-7) infusion in anaesthetized rats

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dc.contributor.author O'Neill, Julie
dc.contributor.author Healy, Vincent
dc.contributor.author Johns, Edward J.
dc.date.accessioned 2018-05-28T11:58:05Z
dc.date.available 2018-05-28T11:58:05Z
dc.date.issued 2017-09-21
dc.identifier.citation O'Neill, J., Healy, V., Johns, E. J. (2017) 'Intrarenal Mas and AT(1) receptors play a role in mediating the excretory actions of renal interstitial angiotensin-(1-7) infusion in anaesthetized rats', Experimental Physiology, 102, pp. 1700-1715. doi:10.1113/EP086513 en
dc.identifier.volume 102 en
dc.identifier.startpage 1700 en
dc.identifier.endpage 1715 en
dc.identifier.issn 0958-0670
dc.identifier.uri http://hdl.handle.net/10468/6198
dc.identifier.doi 10.1113/EP086513
dc.description.abstract New Findings What is the central question of this study? Dietary sodium manipulation alters the magnitude of angiotensin‐(1–7) [Ang‐(1–7)]‐induced natriuresis. The present study sought to determine whether this was related to relative changes in the activity of intrarenal Mas and/or AT1 receptors. What is the main finding and its importance? Angiotensin‐(1–7)‐induced diuresis and natriuresis is mediated by intrarenal Mas receptors. However, intrarenal AT1 receptor blockade also had an inhibitory effect on Ang‐(1–7)‐induced natriuresis and diuresis. Thus, Ang‐(1–7)‐induced increases in sodium and water excretion are dependent upon functional Mas and AT1 receptors. We investigated whether angiotensin‐(1–7) [Ang‐(1–7)]‐induced renal haemodynamic and excretory actions were solely dependent upon intrarenal Mas receptor activation or required functional angiotensin II type 1 (AT1) receptors. The renin–angiotensin system was enhanced in anaesthetized rats by prior manipulation of dietary sodium intake. Angiotensin‐(1–7) and AT1 and Mas receptor antagonists were infused into the kidney at the corticomedullary border. Mas receptor expression was measured in the kidney. Mean arterial pressure, urine flow and fractional sodium excretion were 93 ± 4 mmHg, 46.1 ± 15.7 μl min−1 kg−1 and 1.4 ± 0.3%, respectively, in the normal‐sodium group and 91 ± 2 mmHg, 19.1 ± 3.3 μl min−1 kg−1 and 0.7 ± 0.2%, respectively, in the low‐sodium group. Angiotensin‐(1–7) infusion had no effect on mean arterial pressure in rats receiving a normal‐sodium diet but decreased it by 4 ± 5% in rats receiving a low‐sodium diet (P < 0.05). Interstitial Ang‐(1–7) infusion increased urine flow twofold and fractional sodium excretion threefold (P < 0.05) in rats receiving a normal‐sodium diet and to a greater extent, approximately three‐ and fourfold, respectively, in rats receiving the low‐sodium diet (both P < 0.05). Angiotensin‐(1–7)‐induced increases in urine flow and fractional sodium excretion were absent in both dietary groups during intrarenal AT1 or Mas receptor inhibition after either losartan or A‐779, respectively. Thus, AT1 receptor activation, as well as Mas receptor activation, plays an essential role in mediating Ang‐(1–7)‐induced natriuresis and diuresis. Whether this is because Ang‐(1–7) partly antagonizes AT1 receptors or whether Ang‐(1–7)‐induced natriuresis is mediated through AT1–Mas receptor dimerization remains unclear. en
dc.description.sponsorship Irish Research Council for Science, Engineering and Technology (Grant number R13742) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher John Wiley & Sons, Inc. en
dc.rights © 2017, The Authors. Experimental Physiology ©2017 The Physiological Society. This is the peer reviewed version of the following article: O'Neill, Julie; Healy, Vincent; Johns, Edward J. (2017) 'Intrarenal Mas and AT(1) receptors play a role in mediating the excretory actions of renal interstitial angiotensin-(1-7) infusion in anaesthetized rats'. Experimental Physiology, 102 :1700-1715. doi:10.1113/EP086513, which has been published in final form at doi:10.1113/EP086513. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. en
dc.subject II Type-1 receptor en
dc.subject Natriuretic action en
dc.subject Converting enzyme en
dc.subject Hypertensive rats en
dc.subject Ace inhibition en
dc.subject Responses en
dc.subject Antagonist en
dc.subject Blockade en
dc.subject Ligand en
dc.subject Cortex en
dc.title Intrarenal Mas and AT(1) receptors play a role in mediating the excretory actions of renal interstitial angiotensin-(1-7) infusion in anaesthetized rats en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Vincent Healy, Physiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: v.healy@ucc.ie en
dc.internal.availability Full text available en
dc.check.info Access to this article is restricted until 12 months after publication by request of the publisher en
dc.check.date 2018-09-21
dc.date.updated 2018-05-28T11:35:02Z
dc.description.version Accepted Version en
dc.internal.rssid 421653485
dc.internal.wokid WOS:000416871800011
dc.contributor.funder Irish Research Council for Science, Engineering and Technology en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Experimental Physiology en
dc.internal.copyrightchecked Yes en
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress v.healy@ucc.ie en


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