An investigation of the prebiotic potential and gut health benefits of Irish seaweeds

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dc.contributor.advisor Stanton, Catherine en
dc.contributor.advisor Fitzgerald, Gerald F. en
dc.contributor.advisor Ross, R. Paul en Collins, Kenneth G. 2018-07-31T12:11:50Z 2018-07-31T12:11:50Z 2017 2017
dc.identifier.citation Collins, K. G. 2017. An investigation of the prebiotic potential and gut health benefits of Irish seaweeds. PhD Thesis, University College Cork. en
dc.identifier.endpage 343 en
dc.description.abstract Seaweeds are an excellent source of biologically active secondary metabolites. Compounds from seaweeds have exhibited a wide range of therapeutic activities and seaweeds have now been proposed as a new source of prebiotics. Firstly, the bifidogenic potential of 15 Irish seaweeds was assessed using in vitro anaerobic fermentations. This investigation suggests that different Bifidobacterium species can utilise seaweed carbohydrates as their sole carbon source for growth, with the greatest bifidogenic activity exhibited by Fucus serratus. Following this, the prebiotic potential of F. serratus was investigated using a faecal fermentation approach. An extract was prepared using a hot-acid extraction followed by ethanol precipitation, size-exclusion dialysis and a simulated gastric digestion. While not all the expected prebiotic markers were met, an increase in the production of SCFA, especially propionate, is a positive outcome for promoting overall gut health and in combating obesity through enhancing satiety. Two similarly processed extracts from the seaweed Laminaria digitata were prepared to study the effect of depolymerisation with hydrogen peroxide on prebiotic potential. Depolymerisation significantly increased propionate production and reduced butyrate production. Modulation of polysaccharide chain length can bring about different fermentation profiles from the same seaweed raw material and starting faecal microbiota. The prebiotic potential of the red seaweed Chondrus crispus was also investigated, however, only a minimal stimulatory effect on the ex vivo microbial population was observed and limited changes in SCFA production. Finally, polyphenol rich extracts from Fucus vesiculosus, F. serratus F. spiralis, Ascophyllum nodosum and Pelvetia canaliculata demonstrated potent activity against Listeria monocytogenes. Greatest activity was observed with F. vesiculosus extracts, which showed activity against several strains of Listeria. Overall this thesis shows that seaweed polysaccharides, especially those from brown seaweeds have great prebiotic potential but the definitive prebiotic test will be to transition to in vivo trials in animals and eventually humans. en
dc.description.sponsorship Grant-Aid Agreement No. MFFRI/07/01 en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher University College Cork en
dc.rights © 2017, Kenneth G. Collins. en
dc.rights.uri en
dc.subject Seaweed en
dc.subject Prebiotic en
dc.subject Bifidobacterium en
dc.subject Short-chain fatty acids en
dc.subject Antimicrobial en
dc.subject Depolymerisation en
dc.subject Hot-acid extraction en
dc.subject Microbiota en
dc.subject Dietary fiber en
dc.title An investigation of the prebiotic potential and gut health benefits of Irish seaweeds en
dc.type Doctoral thesis en
dc.type.qualificationlevel Doctoral en
dc.type.qualificationname PhD en
dc.internal.availability Full text available en Not applicable en
dc.description.version Accepted Version
dc.contributor.funder Marine Institute en
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.contributor.funder Teagasc en
dc.description.status Not peer reviewed en Microbiology en
dc.check.type No Embargo Required
dc.check.reason Not applicable en
dc.check.opt-out Not applicable en
dc.thesis.opt-out false
dc.check.embargoformat Embargo not applicable (If you have not submitted an e-thesis or do not want to request an embargo) en
dc.internal.conferring Spring 2018 en
dc.relation.project Department of Agriculture, Food and the Marine (Grant-Aid Agreement No. MFFRI/07/01); Teagasc (Walsh Fellowship Scheme) en

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© 2017, Kenneth G. Collins. Except where otherwise noted, this item's license is described as © 2017, Kenneth G. Collins.
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