Determination optical properties of tissue-like phantoms using diffuse reflectance and transmittance spectroscopy

Abstract

We present a simple, convenient fibre-optics probe system for the determination optical properties of tissue-like liquid phantoms based on diffuse reflectance and transmittance measurements at the wavelength of visible-near-infrared region. The method combined Monte Carlo simulations, multiple polynomial regression and a Newton-Raphson algorithm for solving nonlinear equation system. In the range of optical properties of phantom with the μa at 0 – 0.5 cm-1 and the μs’ at 3 – 45 cm-1, the mean prediction error at 630 nm was 1.91 % for μs’ and 7.51 % for μa. The performance of the method is further tested by predictive error and validated using similar matrix of milk-ink phantoms on reflectance and transmittance.