Expansion and diversification of MFS transporters in Kluyveromyces marxianus

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dc.contributor.author Varela, Javier A.
dc.contributor.author Puricelli, Martina
dc.contributor.author Montini, Noemi
dc.contributor.author Morrissey, John P.
dc.date.accessioned 2019-05-22T11:00:34Z
dc.date.available 2019-05-22T11:00:34Z
dc.date.issued 2019-01-10
dc.identifier.citation Varela, J.A., Puricelli, M., Montini, N. and Morrissey, J.P., 2018. Expansion and Diversification of MFS Transporters in Kluyveromyces marxianus. Frontiers in microbiology, 9, 3330, (15pp). DOI: 10.3389/fmicb.2018.03330 en
dc.identifier.volume 9 en
dc.identifier.startpage 1 en
dc.identifier.endpage 15 en
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10468/7964
dc.identifier.doi 10.3389/fmicb.2018.03330 en
dc.description.abstract In yeasts, proteins of the Major Superfamily Transporter selectively bind and allow the uptake of sugars to permit growth on varied substrates. The genome of brewer’s yeast, Saccharomyces cerevisiae, encodes multiple hexose transporters (Hxt) to transport glucose and other MFS proteins for maltose, galactose, and other monomers. For sugar uptake, the dairy yeast, Kluyveromyces lactis, uses Rag1p for glucose, Hgt1 for glucose and galactose, and Lac12 for lactose. In the related industrial species Kluyveromyces marxianus, there are four genes encoding Lac12-like proteins but only one of them, Lac12, can transport lactose. In this study, which initiated with efforts to investigate possible functions encoded by the additional LAC12 genes in K. marxianus, a genome-wide survey of putative MFS sugar transporters was performed. Unexpectedly, it was found was that the KHT and the HGT genes are present as tandem arrays of five to six copies, with the precise number varying between isolates. Heterologous expression of individual genes in S. cerevisiae and mutagenesis of single and multiple genes in K. marxianus was performed to establish possible substrates for these transporters. The focus was on the sugar galactose since it was already reported in K. lactis that this hexose was a substrate for both Lac12 and Hgt1. It emerged that three of the four copies of Lac12, four Hgt-like proteins and one Kht-like protein have some capacity to transport galactose when expressed in S. cerevisiae and inactivation of all eight genes was required to completely abolish galactose uptake in K. marxianus. Analysis of the amino acid sequence of all known yeast galactose transporters failed to identify common residues that explain the selectivity for galactose. Instead, the capacity to transport galactose has arisen three different times in K. marxianus via polymorphisms in proteins that are probably ancestral glucose transporters. Although, this is analogous to S. cerevisiae, in which Gal2 is related to glucose transporters, there are not conserved amino amino changes, either with Gal2, or among the K. marxianus galactose transporters. The data highlight how gene duplication and functional diversification has provided K. marxianus with versatile capacity to utilise sugars for growth. en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fmicb.2018.03330/full
dc.rights © 2019 Varela, Puricelli, Montini and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) en
dc.rights.uri https://creativecommons.org/licenses/by/4.0/ en
dc.subject Sugar en
dc.subject Major facilitator superfamily en
dc.subject Gene duplication en
dc.subject Genome evolution en
dc.subject RAG1 en
dc.subject KHT2 en
dc.subject KHT1 en
dc.subject HGT1 en
dc.subject Kluyveromyces marxianus en
dc.title Expansion and diversification of MFS transporters in Kluyveromyces marxianus en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother John Morrissey, School of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email: j.morrissey@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder FP7 People: Marie-Curie Actions en
dc.contributor.funder European Union’s Horizon 2020 Framework Programme for Research and Innovation en
dc.contributor.funder European Commission en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Microbiology en
dc.internal.IRISemailaddress j.morrissey@ucc.ie en
dc.identifier.articleid 3330 en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/606795/EU/Yeast Cell Factories: Training Researchers to Apply Modern Post-Genomic Methods In Yeast Biotechnology/YEASTCELL en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::RIA/720824/EU/Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds/CHASSY en

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© 2019 Varela, Puricelli, Montini and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) Except where otherwise noted, this item's license is described as © 2019 Varela, Puricelli, Montini and Morrissey. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)
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