Quorum sensing signalling alters virulence potential and population dynamics in complex microbiome-host interactomes

Show simple item record

dc.contributor.author Reen, F. Jerry
dc.contributor.author Gutiérrez-Barranquero, José A.
dc.contributor.author McCarthy, Ronan R.
dc.contributor.author Woods, David F.
dc.contributor.author Scarciglia, Sara
dc.contributor.author Adams, Claire
dc.contributor.author Fog Nielsen, Kristian
dc.contributor.author Gram, Lone
dc.contributor.author O'Gara, Fergal
dc.date.accessioned 2019-10-14T21:37:23Z
dc.date.available 2019-10-14T21:37:23Z
dc.date.issued 2019-09-11
dc.identifier.citation Reen, F. J., Gutiérrez-Barranquero, J. A., McCarthy, R. R., Woods, D. F., Scarciglia, S., Adams, C., Fog Nielsen, K., Gram, L. and O’Gara, F. (2019) 'Quorum Sensing Signaling Alters Virulence Potential and Population Dynamics in Complex Microbiome-Host Interactomes', Frontiers in Microbiology, 10, 2131 (13pp.). DOI: 10.3389/fmicb.2019.02131 en
dc.identifier.volume 10 en
dc.identifier.startpage 1 en
dc.identifier.endpage 13 en
dc.identifier.issn 1664-302X
dc.identifier.uri http://hdl.handle.net/10468/8751
dc.identifier.doi 10.3389/fmicb.2019.02131 en
dc.description.abstract Despite the discovery of the first N-acyl homoserine lactone (AHL) based quorum sensing (QS) in the marine environment, relatively little is known about the abundance, nature and diversity of AHL QS systems in this diverse ecosystem. Establishing the prevalence and diversity of AHL QS systems and how they may influence population dynamics within the marine ecosystem, may give a greater insight into the evolution of AHLs as signaling molecules in this important and largely unexplored niche. Microbiome profiling of Stelletta normani and BD1268 sponge samples identified several potential QS active genera. Subsequent biosensor-based screening of a library of 650 marine sponge bacterial isolates identified 10 isolates that could activate at least one of three AHL biosensor strains. Each was further validated and profiled by Ultra-High Performance Liquid Chromatography Mass Spectrometry, with AHLs being detected in 8 out of 10 isolate extracts. Co-culture of QS active isolates with S. normani marine sponge samples led to the isolation of genera such as Pseudomonas and Paenibacillus, both of which were low abundance in the S. normani microbiome. Surprisingly however, addition of AHLs to isolates harvested following co-culture did not measurably affect either growth or biofilm of these strains. Addition of supernatants from QS active strains did however impact significantly on biofilm formation of the marine Bacillus sp. CH8a sporeforming strain suggesting a role for QS systems in moderating the microbe-microbe interaction in marine sponges. Genome sequencing and phylogenetic analysis of a QS positive Psychrobacter isolate identified several QS associated systems, although no classical QS synthase gene was identified. The stark contrast between the biodiverse sponge microbiome and the relatively limited diversity that was observed on standard culture media, even in the presence of QS active compounds, serves to underscore the extent of diversity that remains to be brought into culture. en
dc.description.sponsorship Enterprise Ireland (CF-2017-0757-P); Health Research Board/Irish Thoracic Society (MRCG-2018-6, MRCG-2014-6); Science Foundation Ireland (SSPC-2); Irish Research Council for Science, Engineering and Technology (GOIPG/2014/647); Cystic Fibrosis Foundation, United States (OG1710); Agilent technologies (Thought Leader Donation of the UHPLC-QTOF system) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Frontiers Media en
dc.relation.uri https://www.frontiersin.org/articles/10.3389/fmicb.2019.02131/full
dc.rights ©2019 Reen, Gutiérrez-Barranquero, McCarthy, Woods, Scarciglia, Adams, Fog Nielsen, Gram and O’Gara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ en
dc.subject Quorum sensing (QS) en
dc.subject Microbiome en
dc.subject Marine sponge bacteria en
dc.subject Cell-cell communication en
dc.subject Acyl homoserine lactone (AHL en
dc.title Quorum sensing signalling alters virulence potential and population dynamics in complex microbiome-host interactomes en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Fergal O Gara, BIOMERIT Research Centre, School of Microbiology, University College Cork, Cork, Ireland. +353-21-490-3000 Email:f.ogara@ucc.ie en
dc.internal.availability Full text available en
dc.description.version Published Version en
dc.contributor.funder Enterprise Ireland en
dc.contributor.funder Health Research Board en
dc.contributor.funder European Commission en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Irish Research Council for Science, Engineering and Technology en
dc.contributor.funder Cystic Fibrosis Foundation en
dc.contributor.funder Agilent Technologies en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Frontiers in Microbiology en
dc.internal.IRISemailaddress f.ogara@ucc.ie en
dc.identifier.articleid 2131 en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/15/TIDA/2977/IE/Pre-clinical testing of novel fungal biofilm blockers for the medical device sector./ en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMARE en


Files in this item

This item appears in the following Collection(s)

Show simple item record

©2019 Reen, Gutiérrez-Barranquero, McCarthy, Woods, Scarciglia, Adams, Fog Nielsen, Gram and O’Gara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Except where otherwise noted, this item's license is described as ©2019 Reen, Gutiérrez-Barranquero, McCarthy, Woods, Scarciglia, Adams, Fog Nielsen, Gram and O’Gara. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
This website uses cookies. By using this website, you consent to the use of cookies in accordance with the UCC Privacy and Cookies Statement. For more information about cookies and how you can disable them, visit our Privacy and Cookies statement