Dissecting the regulation of bile-induced biofilm formation in Staphylococcus aureus

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dc.contributor.author Ulluwishewa, Dulantha
dc.contributor.author Wang, Liang
dc.contributor.author Pereira, Callen
dc.contributor.author Flynn, Stephanie
dc.contributor.author Cain, Elizabeth
dc.contributor.author Stick, Stephen
dc.contributor.author Reen, F. Jerry
dc.contributor.author Ramsay, Joshua P.
dc.contributor.author O'Gara, Fergal
dc.date.accessioned 2019-10-25T14:25:49Z
dc.date.available 2019-10-25T14:25:49Z
dc.date.issued 2016-08-01
dc.identifier.citation Ulluwishewa, D., Wang, L., Pereira, C., Flynn, S., Cain, E., Stick, S., Reen, F. J., Ramsay, J. P. and O’Gara, F. (2016) 'Dissecting the regulation of bile-induced biofilm formation in Staphylococcus aureus', Microbiology, 162(8), pp. 1398-1406. doi: 10.1099/mic.0.000317 en
dc.identifier.volume 162 en
dc.identifier.issued 8 en
dc.identifier.startpage 1398 en
dc.identifier.endpage 1406 en
dc.identifier.issn 1465-2080
dc.identifier.uri http://hdl.handle.net/10468/8865
dc.identifier.doi 10.1099/mic.0.000317 en
dc.description.abstract Aspiration of bile into the cystic fibrosis (CF) lung has emerged as a prognostic factor for reduced microbial lung biodiversity and the establishment of often fatal, chronic pathogen infections. Staphylococcus aureus is one of the earliest pathogens detected in the lungs of children with CF, and once established as a chronic infection, strategies for its eradication become limited. Several lung pathogens are stimulated to produce biofilms in vitro in the presence of bile. In this study, we further investigated the effects of bile on S. aureus biofilm formation. Most clinical S. aureus strains and the laboratory strain RN4220 were stimulated to form biofilms with sub-inhibitory concentrations of bovine bile. Additionally, we observed bile-induced sensitivity to aminoglycosides, which we exploited in a bursa aurealis transposon screen to isolate mutants reduced in aminoglycoside sensitivity and augmented in bile-induced biofilm formation. We identified five mutants that exhibited hypersensitivity to bile with respect to bile-induced biofilm formation, three of which carried transposon insertions within gene clusters involved in wall teichoic acid (WTA) biosynthesis or transport. Strain TM4 carried an insertion between the divergently oriented tagH and tagG genes, which encode the putative WTA membrane translocation apparatus. Ectopic expression of tagG in TM4 restored a wild-type bile-induced biofilm response, suggesting that reduced translocation of WTA in TM4 induced sensitivity to bile and enhanced the bile-induced biofilm formation response. We propose that WTA may be important for protecting S. aureus against exposure to bile and that bile-induced biofilm formation may be an evolved response to protect cells from bile-induced cell lysis. en
dc.description.sponsorship Curtin University, Australia (Health Science Faculty); Irish Department of Agriculture, Food and the Marine; Food Institutional Research Measure (FIRM/RSF/CoFoRD; FIRM 08/RDC/629; FIRM 1/F009/MabS; FIRM 13/F/516); Irish Research Council for Science, Engineering and Technology (PD/2011/2414; GOIPG/2014/647); Health Research Board/Irish Thoracic Society (MRCG-2014-6); The Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013) en
dc.format.mimetype application/pdf en
dc.language.iso en en
dc.publisher Microbiology Society en
dc.relation.uri https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000317
dc.rights © The Authors 2016. The definitive peer reviewed, edited version of this article is published in Microbiology, 162, 8, 2016, https://doi.org/10.1099/mic.0.000317 en
dc.subject Aminoglycosides en
dc.subject Anti-bacterial agents en
dc.subject ATP-binding cassette transporters en
dc.subject Bile en
dc.subject Biofilms en
dc.subject Cell wall en
dc.subject Cystic fibrosis en
dc.subject DNA transposable elements en
dc.subject Humans en
dc.subject Lung en
dc.subject Microbial sensitivity tests en
dc.subject Protein transport en
dc.subject Staphylococcal infections en
dc.subject Staphylococcus aureus en
dc.subject Teichoic Acids en
dc.subject Cystic fibrosis en
dc.subject Staphylococcus en
dc.subject Gastro-esophageal reflux en
dc.title Dissecting the regulation of bile-induced biofilm formation in Staphylococcus aureus en
dc.type Article (peer-reviewed) en
dc.internal.authorcontactother Fergal O'Gara, Microbiology , University College Cork, Cork, Ireland. +353-21-490-3000 Email: f.ogara@ucc.ie en
dc.internal.availability Full text available en
dc.date.updated 2019-10-25T14:05:52Z
dc.description.version Accepted Version en
dc.internal.rssid 419970637
dc.contributor.funder Curtin University, Australia en
dc.contributor.funder Seventh Framework Programme en
dc.contributor.funder Horizon 2020 en
dc.contributor.funder Science Foundation Ireland en
dc.contributor.funder Department of Agriculture, Food and the Marine en
dc.contributor.funder Food Institutional Research Measure en
dc.contributor.funder Health Research Board en
dc.contributor.funder Irish Thoracic Society en
dc.contributor.funder Marine Institute en
dc.contributor.funder Teagasc en
dc.description.status Peer reviewed en
dc.identifier.journaltitle Microbiology en
dc.internal.copyrightchecked No
dc.internal.licenseacceptance Yes en
dc.internal.IRISemailaddress f.ogara@ucc.ie en
dc.internal.IRISemailaddress j.reen@ucc.ie en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/607786/EU/BluePharmTrain/BLUEPHARMTRAIN en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/312184/EU/Increasing Value and Flow in the Marine Biodiscovery Pipeline/PHARMASEA en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/311975/EU/Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications/MACUMBA en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP1::KBBE/287589/EU/Marine Microbial Biodiversity, Bioinformatics and Biotechnology/MICRO B3 en
dc.relation.project info:eu-repo/grantAgreement/EC/FP7::SP3::PEOPLE/256596/EU/Dissecting the role of a novel transcriptional regulator in microbial-host interactomes./MEXT REGULATION en
dc.relation.project info:eu-repo/grantAgreement/EC/H2020::IA/634486/EU/Industrial Applications of Marine Enzymes: Innovative screening and expression platforms to discover and use the functional protein diversity from the sea/INMARE en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Research Centres/12/RC/2275/IE/Synthesis and Solid State Pharmaceutical Centre (SSPC)/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/13/TIDA/B2625/IE/Small molecule inhibitors of HIF-1: a new class of anti-cancer therapeutics./ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/12/TIDA/B2411/IE/Development of small molecule therapeutics for medical intervention: anti-biofilm inhibitors for the medical device sector./ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/12/TIDA/B2405/IE/Optimised detection of key biomarkers of Pseudomonas aeruginosa towards a clinical application/ en
dc.relation.project info:eu-repo/grantAgreement/SFI/SFI Technology and Innovation Development Award (TIDA)/14/TIDA/2438/IE/Next Generation Antibiotics: anti-biofilm, anti-pathogenic natural bioactives from marine microorganisms./ en

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