Nanofabrication towards biophotonics

dc.check.embargoformatNot applicableen
dc.check.infoNo embargo requireden
dc.check.opt-outNot applicableen
dc.check.reasonNo embargo requireden
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dc.contributor.advisorO'Riordan, Alanen
dc.contributor.advisorLovera, Pierreen
dc.contributor.authorJones, Daniel
dc.contributor.funderEuropean Unionen
dc.contributor.funderScience Foundation Irelanden
dc.contributor.funderHigher Education Authorityen
dc.date.accessioned2016-06-10T10:53:41Z
dc.date.available2016-06-10T10:53:41Z
dc.date.issued2015
dc.date.submitted2015
dc.description.abstractThis thesis explores methods for fabrication of nanohole arrays, and their integration into a benchtop system for use as sensors or anti-counterfeit labels. Chapter 1 gives an introduction to plasmonics and more specifically nanohole arrays and how they have potential as label free sensors compared to the current biosensors on the market. Various fabrication methods are explored, including Focused Ion Beam, Electron Beam Lithography, Nanoimprint lithography, Template stripping and Phase Shift Lithography. Focused Ion Beam was chosen to fabricate the nanohole arrays due to its suitability for rapid prototyping and it’s relatively low cost. In chapter 2 the fabrication of nanohole arrays using FIB is described, and the samples characterised. The fabricated nanohole arrays are tested as bulk refractive index sensors, before a bioassay using whole molecule human IgG antibodies and antigen is developed and performed on the senor. In chapter 3 the fabricated sensors are integrated into a custom built system, capable of real time, multiplexed detection of biomolecules. Here, scFv antibodies of two biomolecules relevant to the detection of pancreatic cancer (C1q and C3) are attached to the nanohole arrays, and detection of their complementary proteins is demonstrated both in buffer (10 nM detection of C1q Ag) and human serum. Chapter 4 explores arrays of anisotropic (elliptical) nanoholes and shows how the shape anisotropy induces polarisation sensitive transmission spectra, in both simulations and fabricated arrays. The potential use of such samples as visible and NIR tag for anti-counterfeiting applications is demonstrated. Finally, chapter 5 gives a summary of the work completed and discusses potential future work in this area.en
dc.description.sponsorshipEuropean Union (EU Framework 7 under the Phast-ID project [258238]); Science Foundation Ireland (EU under US-Ireland Agri-Sense project [12/US/I2476]); Higher Education Authority (PRTLI programs [Cycle 3 Nanoscience and Cycle 4 INSPIRE])en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationJones, D. 2015. Nanofabrication towards biophotonics. PhD Thesis, University College Cork.en
dc.identifier.endpage145en
dc.identifier.urihttps://hdl.handle.net/10468/2720
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2015, Daniel Jones.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectImmuno-sensoren
dc.subjectPlasmonicsen
dc.subjectNanohole arrayen
dc.subjectReal time label-free biosensingen
dc.subjectC1q & C3 antigen detectionen
dc.subjectPancreatic cancer biomarkersen
dc.thesis.opt-outfalse
dc.titleNanofabrication towards biophotonicsen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePHD (Engineering)en
ucc.workflow.supervisoralan.oriordan@tyndall.ie
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