An analysis of selected secretion systems of Pseudomonas species

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dc.check.embargoformatNot applicableen
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dc.contributor.advisorO'Gara, Fergalen
dc.contributor.authorEgan, Michael Frank
dc.contributor.funderHigher Education Authorityen
dc.date.accessioned2016-06-22T09:22:40Z
dc.date.available2016-06-22T09:22:40Z
dc.date.issued2014
dc.date.submitted2014
dc.description.abstractDirect secretion systems which deliver molecules from one cell to another have huge significance in shaping bacterial communities or in determining the outcome of bacterial associations with eukaryotic organisms. This work examines the roles of the Type III Secretion System (T3SS) and the Type VI Secretion System (T6SS) systems of Pseudomonas, a widespread genus including clinical pathogens and biocontrol strains. Bioinformatic analysis of T6SS phylogeny and associated gene content within Pseudomonas identified several T6SS phylogenetic groups, and linked T6SS components VgrG and Hcp encoded outside of T6SS gene loci with their cognate T6SS phylogenetic groups. Remarkably, such “orphan” vgrG and hcp genes were found to occur in diverse, horizontally transferred, operons often containing putative T6SS accessory components and effectors. The prevalence of a widespread superfamily of T6SS lipase effectors (Tle) was assessed in metagenomes from various environments. The abundance of the Tle superfamily and individual families varied between niches, suggesting there is niche specific selection and specialisation of Tle. Experimental work also discovered that P. fluorescens F113 uses the SPI-1 T3SS to avoid amoeboid grazing in mixed populations. This finding may represent a significant aspect of F113 rhizocompetence, and the rhizocompetence of other Rhizobacteria.en
dc.description.sponsorshipHigher Education Authority (PRTLI4)en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationEgan, M.F. 2014. An analysis of selected secretion systems of pseudomonas species. PhD Thesis, University College Corken
dc.identifier.endpage258en
dc.identifier.urihttps://hdl.handle.net/10468/2771
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2014, Michael Frank Egan.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectPseudomonasen
dc.subjectType VI secretion systemen
dc.subjectType III secretion systemen
dc.subjectType VI lipase effectorsen
dc.subjectVgrG islandsen
dc.thesis.opt-outfalse
dc.titleAn analysis of selected secretion systems of Pseudomonas speciesen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD (Science)en
ucc.workflow.supervisorf.ogara@ucc.ie
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