Differences in amino acid digestibility between young and older adults: A randomized crossover study using the dual tracer method

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dc.contributor.authorHinssen, Fenna
dc.contributor.authorTessier, Romain
dc.contributor.authorvan Harskamp, Dewi
dc.contributor.authorHuppertz, Thom
dc.contributor.authorMensink, Marco
dc.contributor.authorvan der Wielen, Nikkie
dc.contributor.funderDutch Ministry of Economic Affairs, LWV20155
dc.contributor.funderNestlé, Dairy Management Inc.
dc.contributor.funderFrieslandCampina
dc.contributor.funderArla Foods amb, LWV20155
dc.date.accessioned2026-10-13T13:15:02Z
dc.date.available2026-10-13T13:15:02Z
dc.date.issued2026-07-09
dc.description.abstractBackground: Older adults may have reduced amino acid (AA) digestibility due to physiological changes in the digestive tract, but quantification of reduced AA digestibility in humans is lacking. Objectives: This randomized crossover study investigated differences in indispensable AA digestibility, primarily focusing on threonine and lysine, between young and older adults for milk, sorghum, and black beans using the dual tracer method. Methods: Ten young (21.8 ± 1.7 y) and 10 older (72.8 ± 3.8 y) adults ingested 20 g 2H-labeled protein from either milk, sorghum, or black beans, mixed with 400 mg of a 13C-labeled AA mixture, in a plateau feeding protocol on 3 separate test days. Blood was sampled before and at regular intervals over an 8-h period after meal consumption. The 2H- and 13C- enrichments of AAs in plasma samples were determined at steady state (5.5–8.0 h). The ratio of the 2H/13C-ratio between blood plasma and test meals was calculated. For lysine and threonine, this ratio was compared statistically between age groups and protein sources using linear mixed model analysis. Results: The isotope plasma-to-meal ratio for lysine and threonine was 19% lower in older adults compared with young adults for sorghum (P = 0.041), whereas it was not significantly different for milk (P = 0.277) and black beans (P = 0.849). Conclusions: Lysine and threonine digestion might be lower in older compared with young adults, but the effect differed among protein sources. Furthermore, research on how aging affects protein digestibility across protein sources is needed to optimize dietary protein recommendations for older adults.This study was registered at clinicaltrials.gov as NCT05534464.en
dc.description.sponsorshipDutch Ministry of Economic Affairs|LWV20155|Quantifying differences in bioavailability of different dietary proteins in older adults
dc.description.sponsorshipNestlé, Dairy Management Inc., FrieslandCampina, Arla Foods amb|LWV20155|Quantifying differences in bioavailability of different dietary proteins in older adults
dc.description.versionPublished Version
dc.format.extent9
dc.format.mimetypeapplication/pdfen
dc.identifier.articleid101361
dc.identifier.authororcidHinssen, Fenna
dc.identifier.authororcidTessier, Romain
dc.identifier.authororcidvan Harskamp, Dewi
dc.identifier.authororcidHuppertz, Thom
dc.identifier.authororcidMensink, Marco
dc.identifier.authororcidvan der Wielen, Nikkie
dc.identifier.citationHinssen, F, Tessier, R, van Harskamp, D, Huppertz, T, Mensink, M & van der Wielen, N 2026, 'Differences in amino acid digestibility between young and older adults: A randomized crossover study using the dual tracer method', American Journal of Clinical Nutrition, vol. 124, no. 1, 101361, pp. 1-9. https://doi.org/10.1016/j.ajcnut.2026.101361
dc.identifier.doi10.1016/j.ajcnut.2026.101361
dc.identifier.endpage9
dc.identifier.issn0002-9165
dc.identifier.issued1
dc.identifier.journaltitleAmerican Journal of Clinical Nutrition
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/10468/19434
dc.identifier.volume124
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.urihttps://www.scopus.com/pages/publications/105040953986
dc.rights© 2026, authors. Published by Elsevier Inc. on behalf of American Society for Nutrition. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/)
dc.rights.accessrightsopen access
dc.rights.licensenameAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.statusPeer reviewed
dc.subjectAging
dc.subjectAmino acid
dc.subjectDigestibility
dc.subjectDual tracer
dc.subjectOlder adults
dc.subjectProtein
dc.subjectStable isotope
dc.subject[FoodNutritionalSciences]
dc.titleDifferences in amino acid digestibility between young and older adults: A randomized crossover study using the dual tracer methoden
dc.typeArticle (peer-reviewed)
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