Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking

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dc.contributor.authorWaschbüsch, Dieter
dc.contributor.authorHübel, Nicole
dc.contributor.authorOssendorf, Edith
dc.contributor.authorLobbestael, Evy
dc.contributor.authorBaekelandt, Veerle
dc.contributor.authorLindsay, Andrew J.
dc.contributor.authorMcCaffrey, Mary W.
dc.contributor.authorKhan, Amir R.
dc.contributor.authorBarnekow, Angelika
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2019-03-28T11:20:49Z
dc.date.available2019-03-28T11:20:49Z
dc.date.issued2019
dc.description.abstractThe Rab family of small GTPases regulate various aspects of cellular dynamics in eukaryotic cells. Membrane trafficking has emerged as central to the functions of leucine-rich repeat kinase 2 (LRRK2), which is associated with inherited and sporadic forms of Parkinson’s disease (PD). Rabs act as both regulators of the catalytic activity and targets for serine/threonine phosphorylation by LRRK2. Rab32, Rab38 and Rab29 have been shown to regulate LRRK2 sub-cellular localization through direct interactions. Recently, Rab29 was shown to escort LRRK2 to the Golgi apparatus and activate the phosphorylation of Rab8 and Rab10. Rab32 is linked to multiple cellular functions including endosomal trafficking, mitochondrial dynamics, and melanosome biogenesis. A missense mutation in Rab32 has also recently been linked to PD. Here, we demonstrate that Rab32 directly interacts with sorting nexin 6 (SNX6). SNX6 is a transient subunit of the retromer, an endosome-Golgi retrieval complex whose Vps35 subunit is strongly associated with PD. We could further show that localization of cation-independent mannose-6-phosphate receptors, which are recycled to the trans-Golgi network (TGN) by the retromer, was affected by both Rab32 and SNX6. These data imply that Rab32 is linked to SNX6/retromer trafficking at the Golgi, and also suggests a possible connection between the retromer and Rab32 in the trafficking and biological functions of LRRK2.en
dc.description.statusPeer revieweden
dc.description.versionPublished Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.articleide0208889
dc.identifier.citationWaschbüsch, D., Hübel, N., Ossendorf, E., Lobbestael, E., Baekelandt, V., Lindsay, A.J., McCaffrey, M.W., Khan, A.R. and Barnekow, A., 2019. Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking. PloS one, 14(1). (e0208889). DOI: 10.1371/journal.pone.0208889en
dc.identifier.doi10.1371/journal.pone.0208889
dc.identifier.endpage18en
dc.identifier.issn1932-6203
dc.identifier.issued1en
dc.identifier.journaltitlePLOS ONEen
dc.identifier.startpage1en
dc.identifier.urihttps://hdl.handle.net/10468/7683
dc.identifier.volume14en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.projectinfo:eu-repo/grantAgreement/SFI/SFI Investigator Programme/12/IA/1239/IE/Molecular Aspects of Immune Evasion and Subversion of Membrane Trafficking by Pathogens/en
dc.relation.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208889
dc.rights© 2019 Waschbüsch et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en
dc.subjectRaben
dc.subjectCellular dynamicsen
dc.subjectMembrane traffickingen
dc.titleRab32 interacts with SNX6 and affects retromer-dependent Golgi traffickingen
dc.typeArticle (peer-reviewed)en
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