The impact of host-microbe interactions on murine colonic secretomotor function

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dc.check.embargoformatNot applicableen
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dc.contributor.advisorHyland, Niall P.en
dc.contributor.advisorCryan, John F.en
dc.contributor.authorLomasney, Kevin W.
dc.contributor.funderScience Foundation Irelanden
dc.date.accessioned2015-10-20T10:45:40Z
dc.date.available2015-10-20T10:45:40Z
dc.date.issued2014
dc.date.submitted2014
dc.description.abstractThe overall objective of this thesis was to gain further insight into the mechanisms underlying commensal microbial influences on intestinal ion transport. In this regard, I examined the impact of commensal host-microbe interactions on colonic secretomotor function in mouse. I first examined the influence of two different probiotic (microorganisms which, when given in adequate amounts, can confer health benefits upon the host) strains, Bifidobacterium infantis 35624 and L. salivarius UCC118 on active colonic ion transport in the mouse, using the Ussing Chamber. I found that both probiotics appear to have converging effects on ion transport at a functional level. However, L. salivarius UCC118 may preferentially inhibit neurally-evoked ion transport. Next I examined the impact of the host microbiota itself on both baseline and stimulated colonic secretomotor function as well as probiotic induced changes in ion transport. I provide further evidence that the microbiota is capable of mediating alterations in colonic ion transport, and specifically suggests that it can influence cAMP-mediated responses. Finally, it has been well documented that many probiotics elicit their effects via secreted bioactives, therefore, I studied the effects of microbially produced GABA, contained in supernatants from the commensal microbe Lactobacillus brevis DPC6108, on colonic secretomotor function. In conclusion, I believe that commensal microbes have an important and strain specific functional influence on colonic ion transport and secretomotor function and these effects can be mediated via extracellular bioactives. Moreover, I believe that functional ex-vivo studies such as those carried out in this thesis have a critical role to play in our future understanding of host-microbe interactions in the gut.en
dc.description.sponsorshipScience Foundation Ireland (Alimentary Pharmabiotic Centre Funding)en
dc.description.statusNot peer revieweden
dc.description.versionAccepted Version
dc.format.mimetypeapplication/pdfen
dc.identifier.citationLomasney, K. W. 2014. The impact of host-microbe interactions on murine colonic secretomotor function. PhD Thesis, University College Cork.en
dc.identifier.endpage206
dc.identifier.urihttps://hdl.handle.net/10468/2002
dc.language.isoenen
dc.publisherUniversity College Corken
dc.rights© 2014, Kevin W. Lomasney.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/en
dc.subjectMicrobiotaen
dc.subjectProbioticsen
dc.subjectIntestineen
dc.subjectMouseen
dc.subjectIon transporten
dc.subjectEnteric nervous systemen
dc.subjectUssing chamberen
dc.thesis.opt-outfalse
dc.titleThe impact of host-microbe interactions on murine colonic secretomotor functionen
dc.typeDoctoral thesisen
dc.type.qualificationlevelDoctoralen
dc.type.qualificationnamePhD (Medicine and Health)en
ucc.workflow.supervisorn.hyland@ucc.ie
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