Bovine mastitis is a polymicrobial disease requiring a polydiagnostic approach

Simple item page
dc.contributor.authorAngelopoulou, Angeliki
dc.contributor.authorHolohan, Ross
dc.contributor.authorRea, Mary C.
dc.contributor.authorWarda, Alicja K.
dc.contributor.authorHill, Colin
dc.contributor.funderScience Foundation Ireland
dc.date.accessioned2024-01-17T14:37:29Z
dc.date.available2024-01-17T14:37:29Z
dc.date.issued2019
dc.description.abstractBovine mastitis, an inflammation of the udder, is associated with increases in milk somatic cell count usually resulting from bacterial infection. We analysed 50 mastitic milk samples via cultivation, 16S rRNA sequencing and a combination of the two (culturomics) to define the complete microbial content of the milk. Most samples contained over 10,000 cfu mL−1 total bacterial counts including isolates that were haemolysin positive (n = 36). Among colonies isolated from blood agar plates, Streptococcus uberis was dominant (11/50) followed by Streptococcus dysgalactiae (6/50), Pseudomonas (6/50), Enterococcus faecalis (6/50), Escherichia coli (6/50), Staphylococcus argenteus (4/50), Bacillus (4/50) and Staphylococcus aureus (2/50). 16S rRNA profiling revealed that amplicons were dominated by Rhodococcus, Staphylococcus, Streptococcus and Pseudomonas. A higher inter-sample diversity was noted in the 16S rRNA readouts, which was not always reflected in the plating results. The combination of the two methods highlights the polymicrobial complexity of bovine mastitis. © 2019 Elsevier Ltden
dc.description.sponsorshipScience Foundation Ireland (SFI/12/RC/2273)en
dc.description.statusPeer revieweden
dc.description.versionAccepted Versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationAngelopoulou, A., Holohan, R., Rea, M. C., Warda, A. K., Hill, C. and Ross, R. P. (2019) 'Bovine mastitis is a polymicrobial disease requiring a polydiagnostic approach', International Dairy Journal, 99, p.104539. https://doi.org/10.1016/j.idairyj.2019.104539en
dc.identifier.doihttps://doi.org/10.1016/j.idairyj.2019.104539
dc.identifier.issn9586946
dc.identifier.journaltitleInternational Dairy Journalen
dc.identifier.urihttps://hdl.handle.net/10468/15405
dc.identifier.volume99
dc.language.isoenen
dc.publisherElsevier Ltden
dc.rights© 2019, Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBovine mastitisen
dc.titleBovine mastitis is a polymicrobial disease requiring a polydiagnostic approachen
dc.typeArticle (peer-reviewed)en
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
main INDA 104539.pdf
Size:
4.48 MB
Format:
Adobe Portable Document Format
Description:
Accepted Version
Download
Collections
Microbiology - Journal Articles